Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,033,383 |
T→A |
L80Q (CTG→CAG) |
trkH → |
K(+) transporter TrkH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,033,383 | 0 | T | A | 100.0%
| 107.3
/ NA
| 31 | L80Q (CTG→CAG) | trkH | K(+) transporter TrkH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (17/14); total (17/14) |
GGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT > NC_000913/4033305‑4033453
|
ggtggCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAgg > 1:731193/1‑81 (MQ=255)
aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc > 2:731202/1‑81 (MQ=255)
aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc > 1:731197/1‑81 (MQ=255)
aCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCg > 2:731205/1‑81 (MQ=255)
ccGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCgg < 1:731157/81‑1 (MQ=255)
aaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcgc < 2:731158/81‑1 (MQ=255)
aaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGT‑GTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCt > 1:731201/1‑81 (MQ=255)
agagAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCt > 1:731203/1‑81 (MQ=255)
gaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTccc > 2:731208/1‑81 (MQ=255)
gaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTccc < 2:731160/81‑1 (MQ=255)
gaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTccc < 2:731159/81‑1 (MQ=255)
aGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCttt > 1:731220/1‑81 (MQ=255)
ggCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCtttt < 2:731161/81‑1 (MQ=255)
aCTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtctt < 1:731162/81‑1 (MQ=255)
tAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTtctc > 1:731206/1‑81 (MQ=255)
aaaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtcttct < 1:731163/79‑1 (MQ=255)
aaaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTtctc < 2:731164/80‑1 (MQ=255)
aaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCg > 2:731200/1‑80 (MQ=255)
cccGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGa > 2:731199/1‑79 (MQ=255)
gTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGcc > 2:731227/1‑81 (MQ=255)
aGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCg > 2:731211/1‑80 (MQ=255)
gggTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAAc < 1:731165/81‑1 (MQ=255)
ttCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCa > 1:731218/1‑81 (MQ=255)
cTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCAc < 2:731166/80‑1 (MQ=255)
gtggtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTAcc < 1:731167/81‑1 (MQ=255)
gtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGa < 2:731169/80‑1 (MQ=255)
gtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGAt < 2:731168/81‑1 (MQ=255)
ttCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGttt < 2:731170/81‑1 (MQ=255)
tCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttt > 2:731229/1‑81 (MQ=255)
ggACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttttt > 2:731222/1‑80 (MQ=255)
ggACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttttt > 1:731249/1‑80 (MQ=255)
|
GGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT > NC_000913/4033305‑4033453
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
