Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,081,536 |
A→C |
F109V (TTC→GTC) |
fdoI ← |
formate dehydrogenase O subunit gamma |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,081,536 | 0 | A | C | 100.0%
| 79.7
/ NA
| 23 | F109V (TTC→GTC) | fdoI | formate dehydrogenase O subunit gamma |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/14); total (9/14) |
CGCCAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGAAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCAT > NC_000913/4081460‑4081610
|
cGCCAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTta < 2:740845/81‑1 (MQ=255)
ccAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTtat > 1:740873/1‑80 (MQ=255)
aTCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGccc < 2:740846/80‑1 (MQ=255)
aCGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGgtg < 1:740847/81‑1 (MQ=255)
cagcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTc < 2:740848/81‑1 (MQ=255)
cagcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTc < 2:740849/81‑1 (MQ=255)
gcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCt > 2:740871/1‑81 (MQ=255)
cagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTc > 2:740872/1‑81 (MQ=255)
cagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTc < 2:740850/81‑1 (MQ=255)
aggaCCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTg < 2:740851/78‑1 (MQ=255)
agAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGtt > 1:740878/1‑80 (MQ=255)
aGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTgacgac > 2:740883/1‑80 (MQ=255)
gAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGAt < 2:740852/81‑1 (MQ=255)
taatCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTAc < 1:740853/81‑1 (MQ=255)
gccgccCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAat < 1:740854/80‑1 (MQ=255)
gcagccCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAat < 2:740855/77‑1 (MQ=255)
cAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATAttcttc < 2:740856/81‑1 (MQ=255)
aaCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCa < 1:740857/81‑1 (MQ=255)
aCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCaa < 2:740858/81‑1 (MQ=255)
cTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTGGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGatat > 2:740876/1‑80 (MQ=255)
tGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATatca > 2:740901/1‑81 (MQ=255)
gACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATatcat > 1:740885/1‑81 (MQ=255)
gACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATatcat > 2:740881/1‑81 (MQ=255)
|
CGCCAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGAAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCAT > NC_000913/4081460‑4081610
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
