Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,084,127 |
(T)5→6 |
coding (1696/3051 nt) |
fdoG ← |
formate dehydrogenase O subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,084,122 | 1 | . | T | 93.9%
| 107.1
/ 1.3
| 33 | coding (1701/3051 nt) | fdoG | formate dehydrogenase O subunit alpha |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (17/14); total (18/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCCAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTG‑TTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATC > NC_000913/4084042‑4084197
|
tGCCAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGATCTTCAGTTTCGACAGACAACCGATCACtttg > 2:741607/1‑81 (MQ=255)
ccAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACtttg‑t < 2:741553/80‑1 (MQ=255)
cAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACtttg‑ttt > 2:741626/1‑81 (MQ=255)
aGTTAGAGGTTGCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGATAGACAACCGATCACtttg‑ttt‑tttg > 2:741622/1‑79 (MQ=255)
ttAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACtttg‑ttt‑tttgtt > 1:741613/1‑77 (MQ=255)
agagGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCg > 1:741612/1‑81 (MQ=255)
agGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCggg < 1:741556/81‑1 (MQ=255)
agGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCggg < 1:741557/81‑1 (MQ=255)
gTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGaa < 2:741558/81‑1 (MQ=255)
tcagtgttcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATg < 2:741559/80‑1 (MQ=255)
tcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCaa > 2:741605/1‑79 (MQ=255)
gTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTaa < 1:741560/81‑1 (MQ=255)
cGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAg > 2:741606/1‑81 (MQ=255)
gATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGc > 1:741604/1‑81 (MQ=255)
tGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGccc > 1:741623/1‑81 (MQ=255)
ggTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCt > 2:741619/1‑81 (MQ=255)
gTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTg > 1:741609/1‑81 (MQ=255)
tGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTgg > 2:741608/1‑81 (MQ=255)
gACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTgg > 2:741616/1‑80 (MQ=255)
aCGAGGAACTTCAGTTTCGACAGACAACCGGTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGc < 2:741561/80‑1 (MQ=38)
cGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAg < 2:741562/81‑1 (MQ=255)
gAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGata > 1:741621/1‑80 (MQ=255)
cTTCAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGc < 1:741563/81‑1 (MQ=255)
cAGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAt > 2:741615/1‑81 (MQ=255)
aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt < 2:741566/81‑1 (MQ=255)
aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 2:741596/1‑81 (MQ=255)
aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt < 1:741565/81‑1 (MQ=255)
aGTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 2:741614/1‑81 (MQ=255)
aGTTTCGACAGACAACCGATCACTTTG‑TTTATTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAt < 2:741564/80‑1 (MQ=255)
aGTTTCGACAGACAACCGATCACTTTG‑TTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 2:741632/1‑80 (MQ=255)
gTTTCGACAGACAACCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt > 1:741625/1‑80 (MQ=255)
aaCCGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCctct < 1:741567/81‑1 (MQ=255)
cGATCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtc < 1:741568/81‑1 (MQ=255)
aTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcat < 1:741569/81‑1 (MQ=255)
aTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcat > 2:741617/1‑81 (MQ=255)
aTCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcat > 2:741634/1‑81 (MQ=255)
tCACTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcatc < 2:741570/81‑1 (MQ=255)
cTTTGTTTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCatcatc > 1:741630/1‑81 (MQ=255)
|
TGCCAGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTG‑TTT‑TTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATC > NC_000913/4084042‑4084197
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
