Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,093,668 |
(T)5→4 |
coding (605/825 nt) |
rhaD ← |
rhamnulose‑1‑phosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,093,664 | 0 | T | . | 100.0%
| 159.6
/ NA
| 35 | coding (609/825 nt) | rhaD | rhamnulose‑1‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (19/16); total (19/16) |
TGTCGATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCAT > NC_000913/4093585‑4093740
|
tgtCGATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGtt > 1:743540/1‑81 (MQ=255)
cGATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGttttg > 1:743545/1‑80 (MQ=255)
gATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGttttg > 2:743551/1‑79 (MQ=255)
gATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGttttg > 2:743549/1‑79 (MQ=255)
gATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGttttg > 1:743532/1‑79 (MQ=255)
aaaCCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCAtct > 1:743535/1‑81 (MQ=255)
aaGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTtgtg < 2:743489/81‑1 (MQ=255)
aaGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTtgtg > 1:743556/1‑81 (MQ=255)
aaGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTtgtg > 2:743547/1‑81 (MQ=255)
tttCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGt < 1:743490/81‑1 (MQ=255)
tCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTc < 2:743491/80‑1 (MQ=255)
tCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCg > 1:743553/1‑81 (MQ=255)
cATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCg < 1:743492/80‑1 (MQ=255)
cATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGc > 2:743537/1‑81 (MQ=255)
aTCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGc < 1:743493/80‑1 (MQ=255)
ccAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTg > 2:743568/1‑81 (MQ=255)
cAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTg < 2:743494/80‑1 (MQ=255)
gCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGcc > 1:743555/1‑81 (MQ=255)
cGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGAtt > 2:743576/1‑81 (MQ=255)
ggTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGAttt > 1:743552/1‑81 (MQ=255)
gTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTc < 1:743495/81‑1 (MQ=255)
gCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTg > 2:743571/1‑81 (MQ=255)
cGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGcc < 2:743497/81‑1 (MQ=255)
cGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGcc < 1:743496/81‑1 (MQ=255)
gAAGACGCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCg > 1:743566/1‑81 (MQ=255)
gCCGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCAcc < 2:743498/81‑1 (MQ=255)
cGTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCAt > 2:743563/1‑81 (MQ=255)
gTGGAAGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATc > 2:743565/1‑81 (MQ=255)
aaGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACg > 2:743561/1‑81 (MQ=255)
aaGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACg < 2:743499/81‑1 (MQ=255)
aGGGCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACgg > 1:743570/1‑81 (MQ=255)
gggCCACAACACCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGc < 1:743500/81‑1 (MQ=255)
acaacaCCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAAt > 2:743543/1‑81 (MQ=255)
caacaCCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATg > 2:743572/1‑81 (MQ=255)
aacaCCAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGc > 2:743564/1‑81 (MQ=255)
ccAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAAc < 1:743501/81‑1 (MQ=255)
ccAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAAc < 2:743502/81‑1 (MQ=255)
cAGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACg < 1:743503/81‑1 (MQ=255)
aGCGAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGc > 2:743569/1‑81 (MQ=255)
gAATG‑TTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACTGCAAAATGCCAACGCCAt < 2:743504/81‑1 (MQ=255)
|
TGTCGATTAAACCGAAGGTTTCATCCAGCGTCGGTCCGCTGCCGAAGACGCCGTGGAAGGGCCACAACACCAGCGAATGTTTTTGCATCTCTTGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCAT > NC_000913/4093585‑4093740
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
