Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,104,429 |
C→T |
A183T (GCC→ACC) |
cpxA ← |
sensory histidine kinase CpxA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,104,429 | 0 | C | T | 100.0%
| 104.2
/ NA
| 30 | A183T (GCC→ACC) | cpxA | sensory histidine kinase CpxA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/16); total (14/16) |
TGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTT > NC_000913/4104353‑4104504
|
tGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAc < 1:745857/81‑1 (MQ=255)
tGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAc < 2:745855/81‑1 (MQ=255)
tGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAc < 1:745856/81‑1 (MQ=255)
ggCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACcac > 2:745897/1‑80 (MQ=255)
aaGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAAcag > 2:745894/1‑80 (MQ=255)
aGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAAcagc < 1:745858/80‑1 (MQ=255)
tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcgg > 1:745901/1‑81 (MQ=255)
tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcg < 2:745859/80‑1 (MQ=255)
cccTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcgg < 2:745860/80‑1 (MQ=255)
cTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGt < 2:745861/81‑1 (MQ=255)
gggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTAc > 2:745882/1‑81 (MQ=255)
ttCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCaa > 2:745895/1‑81 (MQ=255)
ggCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt > 2:745902/1‑81 (MQ=255)
gCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt > 2:745896/1‑80 (MQ=255)
gCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt > 1:745887/1‑80 (MQ=255)
cAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGa < 2:745862/81‑1 (MQ=255)
gCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACa < 2:745863/81‑1 (MQ=255)
tttttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt > 2:745891/1‑80 (MQ=255)
tttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag > 1:745904/1‑81 (MQ=255)
ttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag < 2:745864/80‑1 (MQ=255)
tCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagc < 2:745865/80‑1 (MQ=255)
aGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagcag < 2:745866/80‑1 (MQ=255)
aGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagcag > 1:745900/1‑80 (MQ=255)
aGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGt < 1:745867/81‑1 (MQ=255)
ttACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa > 2:745889/1‑80 (MQ=255)
cgcCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgg < 2:745868/81‑1 (MQ=255)
ggTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgggcgg < 1:745869/81‑1 (MQ=255)
ttGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCa > 1:745899/1‑80 (MQ=255)
aCTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGt > 2:745910/1‑79 (MQ=255)
cAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGtt < 1:745870/80‑1 (MQ=255)
|
TGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTT > NC_000913/4104353‑4104504
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
