Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,104,429 |
C→T |
A183T (GCC→ACC) |
cpxA ← |
sensory histidine kinase CpxA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,104,429 | 0 | C | T | 100.0%
| 157.2
/ NA
| 43 | A183T (GCC→ACC) | cpxA | sensory histidine kinase CpxA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/21); total (22/21) |
CTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTA > NC_000913/4104355‑4104505
|
cTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAcc < 2:810695/80‑1 (MQ=255)
gCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCacaa < 1:810696/81‑1 (MQ=255)
cGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCacaac < 2:810697/81‑1 (MQ=255)
gTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCacaa > 1:810741/1‑79 (MQ=255)
aaGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAAcag > 2:810745/1‑80 (MQ=255)
tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcgg > 2:810756/1‑81 (MQ=255)
ccTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcggc < 1:810698/80‑1 (MQ=255)
cTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGt < 2:810699/81‑1 (MQ=255)
gggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTAc > 1:810735/1‑81 (MQ=255)
ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTa < 1:810701/79‑1 (MQ=255)
ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTa < 1:810703/79‑1 (MQ=255)
ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTa < 2:810700/79‑1 (MQ=255)
ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTAc < 2:810702/80‑1 (MQ=255)
gCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTg < 2:810704/81‑1 (MQ=255)
cATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACa > 2:810754/1‑81 (MQ=255)
cATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACa > 2:810749/1‑81 (MQ=255)
aTCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACAt > 1:810750/1‑81 (MQ=255)
cGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATgg > 1:810746/1‑81 (MQ=255)
ggCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt > 2:810740/1‑81 (MQ=255)
ggCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt < 1:810705/81‑1 (MQ=255)
cAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGa > 1:810731/1‑81 (MQ=255)
aGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGAc < 2:810706/81‑1 (MQ=255)
cGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACaa > 2:810737/1‑81 (MQ=255)
gTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt > 2:810707/1‑81 (MQ=255)
tttttCAGCTTACGCGCCGGTTTTACCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATc < 2:810708/81‑1 (MQ=255)
tCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagc < 1:810709/80‑1 (MQ=255)
tCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag > 2:810736/1‑79 (MQ=255)
aGCTTACGCGCCTGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagcag < 2:810710/80‑1 (MQ=255)
gCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGt > 1:810753/1‑80 (MQ=255)
tACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa > 1:810758/1‑79 (MQ=255)
aCGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAg > 2:810767/1‑81 (MQ=255)
gcgcCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcg < 1:810711/81‑1 (MQ=255)
gcCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcggg > 1:810761/1‑81 (MQ=255)
ccGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgggc < 1:810712/81‑1 (MQ=255)
ggTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgggcgg > 2:810738/1‑81 (MQ=255)
ttGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCa > 2:810751/1‑80 (MQ=255)
gCCAGACTCCAGGTCAACCACAACAGCAGCAGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCaaa < 2:810713/80‑1 (MQ=255)
ccAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAAc < 1:810714/80‑1 (MQ=255)
cAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAg > 2:810747/1‑81 (MQ=255)
cAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAg > 2:810748/1‑81 (MQ=255)
ccAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGt > 2:810764/1‑80 (MQ=255)
cAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGtt < 2:810716/80‑1 (MQ=255)
cAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTa < 2:810715/81‑1 (MQ=255)
|
CTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTA > NC_000913/4104355‑4104505
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
