Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,144,842 |
C→T |
S283L (TCA→TTA) |
pflD → |
putative formate acetyltransferase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,144,842 | 0 | C | T | 100.0%
| 124.0
/ NA
| 36 | S283L (TCA→TTA) | pflD | putative formate acetyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/21); total (15/21) |
CTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACAT > NC_000913/4144766‑4144914
|
cTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACattatt > 1:754610/1‑80 (MQ=255)
cTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACattatt < 1:754569/80‑1 (MQ=255)
tGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTaa > 2:754611/1‑81 (MQ=255)
gCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAAc > 1:754615/1‑81 (MQ=255)
cAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTaa < 2:754571/79‑1 (MQ=255)
cAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAAc < 1:754570/80‑1 (MQ=255)
aTACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAAcc > 1:754626/1‑79 (MQ=255)
aTACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCa < 1:754573/81‑1 (MQ=255)
aTACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCa < 2:754572/81‑1 (MQ=255)
tACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAg > 2:754625/1‑81 (MQ=255)
tACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAg < 1:754574/81‑1 (MQ=255)
cGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAggg < 2:754575/81‑1 (MQ=255)
ccAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCg > 2:754613/1‑78 (MQ=255)
aaCGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGAt < 1:754576/81‑1 (MQ=255)
cGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATgc < 1:754577/81‑1 (MQ=255)
aGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATgcggcg > 2:754618/1‑81 (MQ=255)
ttCGCTATCGTTGGGGCCCCTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGtt < 2:754578/81‑1 (MQ=255)
tCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTc > 1:754619/1‑81 (MQ=255)
tCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTc > 1:754627/1‑81 (MQ=255)
tCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGaa < 2:754579/80‑1 (MQ=255)
gTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAg < 2:754580/80‑1 (MQ=255)
ggggCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAAc < 2:754581/80‑1 (MQ=255)
gggCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTg > 1:754612/1‑81 (MQ=255)
gcTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCg < 2:754582/81‑1 (MQ=255)
cTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCg > 1:754622/1‑80 (MQ=255)
cGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATc > 2:754628/1‑81 (MQ=255)
cAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTa < 2:754583/81‑1 (MQ=255)
gTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGa > 2:754620/1‑80 (MQ=255)
ccGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGc < 1:754585/81‑1 (MQ=255)
ccGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGc < 1:754584/81‑1 (MQ=255)
gTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCaa > 1:754647/1‑81 (MQ=255)
gTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCaa > 1:754643/1‑81 (MQ=255)
cTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAAATGCTCGAATCTTTATGGGTGAAATGCAACGa > 2:754624/1‑81 (MQ=255)
tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGa < 1:754589/78‑1 (MQ=255)
tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGAc < 1:754587/79‑1 (MQ=255)
tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACa < 1:754590/80‑1 (MQ=255)
tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACa < 1:754586/80‑1 (MQ=255)
tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACAt < 2:754588/81‑1 (MQ=255)
|
CTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACAT > NC_000913/4144766‑4144914
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
