Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	315	33.5	1889570	98.7%	1865005	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,086	G→A	S25N (AGC→AAC)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,086	0	G	A	100.0%	199.3 / NA	54	S25N (AGC→AAC)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/29); total (25/29)

GCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGATGAG  >  NC_000913/4156010‑4156162

gCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCa                                                                          >  2:823431/1‑81 (MQ=255)

      atgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGt                                                                     >  2:823430/1‑80 (MQ=255)

      atgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGt                                                                     <  2:823380/80‑1 (MQ=255)

      atgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGt                                                                     <  2:823379/80‑1 (MQ=255)

      atgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTg                                                                    <  2:823381/81‑1 (MQ=255)

       tgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGa                                                                   <  1:823383/81‑1 (MQ=255)

       tgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGa                                                                   <  1:823382/81‑1 (MQ=255)

        gaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGaa                                                                  >  1:823432/1‑81 (MQ=255)

          aTCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAAt                                                                 >  1:823420/1‑80 (MQ=255)

           tCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTa                                                               <  1:823385/81‑1 (MQ=255)

           tCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTa                                                               <  2:823384/81‑1 (MQ=255)

               ttaattaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGt                                                           >  1:823451/1‑81 (MQ=255)

                 aattaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTg                                                          <  2:823387/80‑1 (MQ=255)

                 aattaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTg                                                          <  1:823386/80‑1 (MQ=255)

                  attaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAg                                                        <  2:823388/81‑1 (MQ=255)

                     aTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTca                                                     >  1:823389/1‑81 (MQ=255)

                       cAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTcaca                                                   >  2:823439/1‑81 (MQ=255)

                        aaaCTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACAt                                                  >  1:823449/1‑81 (MQ=255)

                         aaCTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACAt                                                  >  2:823436/1‑80 (MQ=255)

                              ggcggcGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGc                                              <  1:823390/79‑1 (MQ=255)

                              ggcggcGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGc                                              <  2:823391/79‑1 (MQ=255)

                               gcggcgTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTc                                           <  1:823392/81‑1 (MQ=255)

                                cggcgTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTcc                                          >  1:823442/1‑81 (MQ=255)

                                 ggcgTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCg                                         >  2:823441/1‑81 (MQ=255)

                                 ggcgTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCg                                         <  1:823393/81‑1 (MQ=255)

                                   cgTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCt                                       >  1:823446/1‑81 (MQ=255)

                                      aCTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGt                                    >  1:823444/1‑81 (MQ=255)

                                      aCTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGt                                    >  2:823445/1‑81 (MQ=255)

                                       ctgctgGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGt                                    >  2:823435/1‑80 (MQ=255)

                                             gATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATtgtg                             <  2:823394/81‑1 (MQ=255)

                                                 gTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcg                         <  2:823395/81‑1 (MQ=255)

                                                 gTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcg                         <  1:823396/81‑1 (MQ=255)

                                                 gTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAc                          <  1:823397/80‑1 (MQ=255)

                                                   gAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggc                       >  1:823433/1‑81 (MQ=255)

                                                    aaGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggc                       <  2:823398/80‑1 (MQ=255)

                                                    aaGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggc                       <  2:823399/80‑1 (MQ=255)

                                                      gagGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggcggc                    >  2:823452/1‑81 (MQ=255)

                                                      gagGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggcggc                    <  2:823400/81‑1 (MQ=255)

                                                      gagGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggcggc                    >  2:823443/1‑81 (MQ=255)

                                                       agGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggcggcg                   >  2:823464/1‑81 (MQ=255)

                                                           gcTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTg               >  2:823466/1‑81 (MQ=255)

                                                           gcTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTg               >  1:823448/1‑81 (MQ=255)

                                                               gAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCgtg           <  1:823402/81‑1 (MQ=255)

                                                               gAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCg             <  2:823401/79‑1 (MQ=255)

                                                                  cGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCgtggtg        <  1:823403/81‑1 (MQ=255)

                                                                   gTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCgtggtg        >  1:823453/1‑80 (MQ=255)

                                                                   gTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGtggtgg       <  2:823404/81‑1 (MQ=255)

                                                                    tCTGTTTAACGCACTGGTGAATTATCGTGGGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGa      >  1:823440/1‑81 (MQ=255)

                                                                    tCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGa      <  1:823405/81‑1 (MQ=255)

                                                                     cTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAt     >  2:823447/1‑81 (MQ=255)

                                                                     cTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAt     >  1:823456/1‑81 (MQ=255)

                                                                        tttAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAt     <  2:823407/78‑1 (MQ=255)

                                                                        tttAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAt     <  1:823408/78‑1 (MQ=255)

                                                                        tttAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGATGAg  <  2:823406/81‑1 (MQ=255)

GCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGATGAG  >  NC_000913/4156010‑4156162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A