Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,162,833 |
C→T |
Q146Q (CAG→CAA) |
trmA ← |
tRNA m(5)U54 methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,162,833 | 0 | C | T | 100.0%
| 110.9
/ NA
| 33 | Q146Q (CAG→CAA) | trmA | tRNA m(5)U54 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/8); total (25/8) |
CGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGG > NC_000913/4162766‑4162908
|
cGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTg > 2:758636/1‑81 (MQ=255)
tAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATcg > 1:758628/1‑81 (MQ=255)
ccTGATCCAGCTCGATTTTGGTTTTAGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATcgcg > 2:758626/1‑79 (MQ=255)
gATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAggg > 2:758627/1‑81 (MQ=255)
tCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGcc > 1:758599/1‑81 (MQ=255)
ccAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCt > 2:758637/1‑81 (MQ=255)
cAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTc < 2:758600/81‑1 (MQ=255)
aGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTcc > 1:758630/1‑81 (MQ=255)
gCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCg > 2:758638/1‑81 (MQ=255)
tCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCt > 1:758639/1‑81 (MQ=255)
cGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCtt > 2:758625/1‑81 (MQ=255)
gATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTc > 1:758646/1‑81 (MQ=255)
aTTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTcc > 1:758648/1‑81 (MQ=255)
aTTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTcc > 1:758601/1‑81 (MQ=255)
ttttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCt < 1:758602/81‑1 (MQ=255)
ttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTg < 2:758603/80‑1 (MQ=255)
gTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGc < 1:758604/81‑1 (MQ=255)
ttttCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGcc < 2:758605/81‑1 (MQ=255)
ttCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCa < 1:758606/80‑1 (MQ=255)
tCGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACt > 2:758644/1‑81 (MQ=255)
cGTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACt > 2:758642/1‑80 (MQ=255)
gTTGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtca > 2:758634/1‑81 (MQ=255)
tGCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtcat > 2:758631/1‑80 (MQ=255)
gCCCGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtcatca > 1:758643/1‑81 (MQ=255)
cGACCAATCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATcc < 1:758607/81‑1 (MQ=255)
aTCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttc > 1:758645/1‑81 (MQ=255)
aTCAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttc < 1:758608/81‑1 (MQ=255)
cAGATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttct > 1:758649/1‑80 (MQ=255)
gATGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTAt > 2:758640/1‑81 (MQ=255)
aTGCACATTCAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATg > 2:758651/1‑81 (MQ=255)
cacaTTCAGATTTTGCGCGCGCAGTGCATCGCGCAGAGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGta > 1:758656/1‑81 (MQ=255)
cAGATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAg > 1:758647/1‑81 (MQ=255)
gATTTTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg > 1:758653/1‑80 (MQ=255)
|
CGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGG > NC_000913/4162766‑4162908
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
