Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,177,755 |
C→T |
P5S (CCT→TCT) |
nusG → |
transcription termination factor NusG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,177,755 | 0 | C | T | 100.0%
| 148.4
/ NA
| 41 | P5S (CCT→TCT) | nusG | transcription termination factor NusG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/19); total (22/19) |
CCTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATC > NC_000913/4177678‑4177832
|
ccTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCt > 1:828424/1‑80 (MQ=255)
cTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTa < 2:828378/80‑1 (MQ=255)
tgGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTaaa < 2:828379/79‑1 (MQ=255)
gggACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAgc < 2:828380/81‑1 (MQ=255)
aTGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTAc < 2:828381/81‑1 (MQ=255)
ggTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTAcgt > 1:828429/1‑81 (MQ=255)
gTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTAcgtc > 2:828421/1‑81 (MQ=255)
ttCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTAcgtcgt < 2:828383/80‑1 (MQ=255)
ttCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGtt < 1:828382/81‑1 (MQ=255)
cTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTc < 1:828384/80‑1 (MQ=255)
tGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAg > 1:828423/1‑81 (MQ=255)
gTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGc > 1:828419/1‑81 (MQ=255)
gCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGttt > 1:828422/1‑81 (MQ=255)
cTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTc < 1:828385/81‑1 (MQ=255)
cTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTc > 1:828448/1‑81 (MQ=255)
tGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTcc > 1:828455/1‑81 (MQ=255)
tCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGttt < 2:828386/81‑1 (MQ=255)
cTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCcgc < 1:828387/81‑1 (MQ=255)
cTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAg > 1:828427/1‑80 (MQ=255)
aGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCaa < 2:828390/80‑1 (MQ=255)
aGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCa < 2:828388/79‑1 (MQ=255)
aGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAAc > 1:828445/1‑81 (MQ=255)
aGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAAc < 2:828389/81‑1 (MQ=255)
tCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTCTTGAAGGCCGCGTAGCAACGTCg > 2:828433/1‑81 (MQ=255)
cTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGc > 1:828446/1‑81 (MQ=255)
tGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCt > 2:828432/1‑81 (MQ=255)
gagaTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTg > 1:828425/1‑81 (MQ=255)
agaTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCt < 1:828391/79‑1 (MQ=255)
aTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGt > 2:828426/1‑81 (MQ=255)
tCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGa < 2:828392/80‑1 (MQ=255)
cTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAg > 2:828443/1‑80 (MQ=255)
cTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAg > 1:828393/1‑80 (MQ=255)
tGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCa > 2:828437/1‑81 (MQ=255)
gAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCa > 1:828449/1‑80 (MQ=255)
aaGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCata < 2:828395/81‑1 (MQ=255)
aaGCTTCTAAAAAGCGCTGGTACGGCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCa < 1:828394/79‑1 (MQ=255)
aGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCatat > 2:828463/1‑81 (MQ=255)
gCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCatat > 1:828428/1‑80 (MQ=255)
gCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCatat > 2:828434/1‑80 (MQ=255)
gCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATc < 1:828397/81‑1 (MQ=255)
gCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATc < 1:828396/81‑1 (MQ=255)
|
CCTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATC > NC_000913/4177678‑4177832
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
