Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,177,755 |
C→T |
P5S (CCT→TCT) |
nusG → |
transcription termination factor NusG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,177,755 | 0 | C | T | 100.0%
| 107.9
/ NA
| 32 | P5S (CCT→TCT) | nusG | transcription termination factor NusG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16) |
CCTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCAT > NC_000913/4177678‑4177829
|
ccTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCt > 2:761584/1‑80 (MQ=255)
gACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAgcgc < 2:761547/81‑1 (MQ=255)
gACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAgcgc > 1:761609/1‑81 (MQ=255)
aCTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCt < 2:767363/81‑1 (MQ=255)
cTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTg > 2:761577/1‑81 (MQ=255)
cTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTg > 1:761587/1‑81 (MQ=255)
gATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTa > 2:761581/1‑81 (MQ=255)
ttCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGtt < 1:761548/81‑1 (MQ=255)
ggTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGATGCTTCTAAAAAGCGCTGGTACGTCGTTCAgg > 2:761595/1‑81 (MQ=255)
gTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAgg < 1:761549/80‑1 (MQ=255)
gTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGc > 1:761606/1‑81 (MQ=255)
gCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGttt > 2:761590/1‑81 (MQ=255)
gCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGtt < 2:761550/80‑1 (MQ=255)
tGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTcc > 2:761600/1‑81 (MQ=255)
gTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTcc < 2:761551/79‑1 (MQ=255)
tATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGt > 1:761611/1‑81 (MQ=255)
aTCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGtt > 2:761599/1‑81 (MQ=255)
aTCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAg < 1:761552/80‑1 (MQ=255)
ccTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAg < 2:761553/81‑1 (MQ=255)
ccTGAGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAg > 2:761619/1‑81 (MQ=255)
aGGTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCaa < 1:761554/80‑1 (MQ=255)
gTTCTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGt < 1:761555/81‑1 (MQ=255)
cTGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCg < 1:761556/80‑1 (MQ=255)
tGAGATGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCt > 2:761593/1‑81 (MQ=255)
gagaTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTg > 2:761602/1‑81 (MQ=255)
agaTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGc < 1:761557/81‑1 (MQ=255)
gaTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCg < 2:761558/81‑1 (MQ=255)
aTGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGt < 2:761559/81‑1 (MQ=255)
tGTCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGt > 1:761605/1‑80 (MQ=255)
tCTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGa < 2:761560/80‑1 (MQ=255)
cTGAAGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAg > 2:761596/1‑80 (MQ=255)
aaGCTTCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCat < 2:761561/80‑1 (MQ=255)
|
CCTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTATCACTGGCCTGAGGTTCTGAGATGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCAT > NC_000913/4177678‑4177829
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
