Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,140 |
A→G |
I966V (ATC→GTC) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,140 | 0 | A | G | 100.0%
| 106.8
/ NA
| 30 | I966V (ATC→GTC) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/16); total (14/16) |
AAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > NC_000913/4184064‑4184213
|
aaaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCt < 1:762975/81‑1 (MQ=255)
aaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc < 1:762976/81‑1 (MQ=255)
aGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGa < 1:762977/81‑1 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCg > 1:763022/1‑81 (MQ=255)
gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTg > 1:763012/1‑81 (MQ=255)
cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGt < 2:762978/81‑1 (MQ=255)
cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGt > 2:763024/1‑81 (MQ=255)
gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGtt < 1:762979/81‑1 (MQ=255)
cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTc < 1:762980/81‑1 (MQ=255)
aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCg < 2:762981/81‑1 (MQ=255)
tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTa < 1:762982/81‑1 (MQ=255)
gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgt > 2:763020/1‑81 (MQ=255)
aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtg > 1:763042/1‑81 (MQ=255)
aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc < 2:762983/81‑1 (MQ=255)
aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc > 2:763025/1‑81 (MQ=255)
aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc < 1:762984/81‑1 (MQ=255)
aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc < 1:762985/81‑1 (MQ=255)
gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct > 2:763015/1‑81 (MQ=255)
gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct < 2:762986/81‑1 (MQ=255)
aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt < 1:762987/80‑1 (MQ=255)
ggCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgg > 1:763033/1‑81 (MQ=255)
gCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt > 2:763038/1‑81 (MQ=255)
gCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt < 2:762988/81‑1 (MQ=255)
cGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg > 1:763041/1‑81 (MQ=255)
agaaagaCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg > 1:763018/1‑81 (MQ=255)
aCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 1:763029/1‑81 (MQ=255)
tCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag < 1:762989/81‑1 (MQ=255)
agaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt < 1:762990/81‑1 (MQ=255)
gaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc > 1:763027/1‑81 (MQ=255)
cTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 1:763046/1‑80 (MQ=255)
|
AAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > NC_000913/4184064‑4184213
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
