Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,140 |
A→G |
I966V (ATC→GTC) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,140 | 0 | A | G | 100.0%
| 158.5
/ NA
| 43 | I966V (ATC→GTC) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/21); total (22/21) |
GAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACA > NC_000913/4184062‑4184215
|
gAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTc < 1:829973/81‑1 (MQ=255)
aaaaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTcc < 1:829974/81‑1 (MQ=255)
aaaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCt > 2:830012/1‑81 (MQ=255)
aaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc < 1:829975/81‑1 (MQ=255)
aaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc < 1:829976/81‑1 (MQ=255)
aaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc > 1:830035/1‑81 (MQ=255)
aaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc > 2:830016/1‑80 (MQ=255)
aaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc > 1:830011/1‑80 (MQ=255)
gACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCg > 1:830030/1‑79 (MQ=255)
aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCgg < 1:829978/81‑1 (MQ=255)
aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCgg < 2:829977/81‑1 (MQ=255)
cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGtt > 2:830021/1‑80 (MQ=255)
cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGtt > 1:830026/1‑80 (MQ=255)
cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTACTCGAAGCGGGTCTGTTc < 2:829979/81‑1 (MQ=255)
tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGtt > 1:830037/1‑79 (MQ=255)
aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGcc < 2:829980/81‑1 (MQ=255)
gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATc < 2:829981/81‑1 (MQ=255)
aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtg < 1:829982/81‑1 (MQ=255)
aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtg > 2:830015/1‑81 (MQ=255)
aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc > 2:830031/1‑81 (MQ=255)
aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgct > 2:830048/1‑81 (MQ=255)
gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt > 1:830029/1‑81 (MQ=255)
cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg > 1:830039/1‑81 (MQ=255)
cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg < 2:829983/81‑1 (MQ=255)
aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc > 2:830038/1‑81 (MQ=255)
gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct < 1:829984/81‑1 (MQ=255)
aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa < 2:829985/81‑1 (MQ=255)
aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg > 1:830032/1‑81 (MQ=255)
aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc > 1:830023/1‑81 (MQ=255)
aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg < 1:829986/81‑1 (MQ=255)
gaagaaAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg < 1:829987/81‑1 (MQ=255)
aagaaagaCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc > 2:830042/1‑81 (MQ=255)
aaagaCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGtt > 1:830028/1‑81 (MQ=255)
agaCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg > 1:830033/1‑80 (MQ=255)
aCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 1:830041/1‑81 (MQ=255)
ccTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc < 1:829988/81‑1 (MQ=255)
cTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt > 1:830046/1‑81 (MQ=255)
gaagaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg > 2:830044/1‑81 (MQ=255)
aagaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga < 2:829989/78‑1 (MQ=255)
gaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc < 2:829991/81‑1 (MQ=255)
gaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc < 1:829990/81‑1 (MQ=255)
aCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa < 1:829992/81‑1 (MQ=255)
cAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACa < 2:829993/79‑1 (MQ=255)
|
GAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACA > NC_000913/4184062‑4184215
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
