Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,293,531 |
G→A |
V123V (GTG→GTA) |
nrfF → |
putative formate‑dependent nitrite reductase complex subunit NrfF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,293,531 | 0 | G | A | 100.0%
| 128.4
/ NA
| 37 | V123V (GTG→GTA) | nrfF | putative formate‑dependent nitrite reductase complex subunit NrfF |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/16); total (21/16) |
GTTAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTC > NC_000913/4293453‑4293605
|
gTTAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAg > 1:786571/1‑81 (MQ=255)
gTTAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAg > 2:786557/1‑81 (MQ=255)
gTTAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAg > 1:786561/1‑81 (MQ=255)
ttAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAgg > 2:786552/1‑81 (MQ=255)
tAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAggg > 2:786562/1‑81 (MQ=255)
aaCGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGc > 2:786572/1‑81 (MQ=255)
aCGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGc < 1:786521/80‑1 (MQ=255)
aCGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGc < 2:786520/80‑1 (MQ=255)
cGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCg > 2:786573/1‑80 (MQ=255)
ggTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAg > 1:786558/1‑81 (MQ=255)
tCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCg < 2:786522/81‑1 (MQ=255)
cAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGa > 1:786585/1‑81 (MQ=255)
gCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGaaa < 2:786523/81‑1 (MQ=255)
tGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGaaa > 1:786560/1‑79 (MQ=255)
ttATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGccc < 2:786524/80‑1 (MQ=255)
aTGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCaa > 1:786566/1‑80 (MQ=255)
tGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCaaaa < 2:786525/81‑1 (MQ=255)
ggtgTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGTAACAGCCCAAAATACCGGTGAAAATg > 1:786577/1‑81 (MQ=255)
tgttgtTACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCt < 1:786526/81‑1 (MQ=255)
gttgttACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCt > 2:786582/1‑80 (MQ=255)
ttgttACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTg < 2:786527/80‑1 (MQ=255)
gttACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGac < 1:786528/80‑1 (MQ=255)
tACTGCTGATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGacaa > 1:786570/1‑80 (MQ=255)
ctgctgATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAacac < 2:786529/81‑1 (MQ=255)
gctgATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACt > 1:786579/1‑80 (MQ=255)
ctgATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACt < 1:786530/79‑1 (MQ=255)
ctgATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTc < 2:786531/80‑1 (MQ=255)
gATGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCAc > 2:786586/1‑80 (MQ=255)
aTGGCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACg < 1:786532/80‑1 (MQ=255)
ggCACTGATCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGata > 2:786578/1‑81 (MQ=255)
tCCTCTGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATggc < 2:786533/81‑2 (MQ=255)
tctGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAtt > 2:786568/1‑81 (MQ=255)
tctGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAtt > 2:786584/1‑81 (MQ=255)
ctGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAttt > 2:786596/1‑81 (MQ=255)
tGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAtt < 1:786534/79‑1 (MQ=255)
gCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTtctc > 2:786575/1‑81 (MQ=255)
gCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTtc < 1:786535/79‑1 (MQ=255)
|
GTTAACGGGTCAGACGCTGGTGTTATGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTC > NC_000913/4293453‑4293605
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
