Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,293,598 |
T→C |
V19A (GTA→GCA) |
nrfG → |
putative formate‑dependent nitrite reductase complex subunit NrfG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,293,598 | 0 | T | C | 100.0%
| 114.6
/ NA
| 32 | V19A (GTA→GCA) | nrfG | putative formate‑dependent nitrite reductase complex subunit NrfG |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/20); total (12/20) |
TCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATC > NC_000913/4293521‑4293675
|
tctGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAtt > 2:786584/1‑81 (MQ=255)
tctGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAtt > 2:786568/1‑81 (MQ=255)
ctGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAttt > 2:786596/1‑81 (MQ=255)
tGGCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCAtt < 1:786534/79‑1 (MQ=255)
gCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTtctc > 2:786575/1‑81 (MQ=255)
gCGAGTAAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTtc < 1:786535/79‑1 (MQ=255)
aGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCg < 2:786536/81‑1 (MQ=255)
gCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCa < 2:786537/81‑1 (MQ=255)
aaGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGtt < 1:786538/81‑1 (MQ=255)
gCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTAt < 1:786539/81‑1 (MQ=255)
cGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATc < 1:786540/81‑1 (MQ=255)
cAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGc > 1:786590/1‑81 (MQ=255)
aGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGcc > 2:786583/1‑81 (MQ=255)
cGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAgg > 1:786580/1‑81 (MQ=255)
cGGTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAgg < 2:786541/81‑1 (MQ=255)
ggTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGc < 1:786543/81‑1 (MQ=255)
ggTGAAAATGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGc < 1:786542/81‑1 (MQ=255)
aaaaTGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGt < 2:786544/80‑1 (MQ=255)
aaaTGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTAc < 1:786545/81‑1 (MQ=255)
aaTGCTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACg > 1:786592/1‑81 (MQ=255)
cTGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGc < 1:786546/80‑1 (MQ=255)
tGACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCg > 2:786598/1‑80 (MQ=255)
gACAACACTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGct > 2:786594/1‑78 (MQ=255)
acTCACGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTAtca < 1:786547/81‑1 (MQ=255)
aCGATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTAtcagcgt < 1:786548/81‑1 (MQ=255)
gATATTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTAtcagcgtca < 2:786549/81‑1 (MQ=255)
tatTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTAtcagcgtcagc < 2:786550/81‑1 (MQ=255)
tatTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTAtcagcgtcagc < 2:786551/81‑1 (MQ=255)
tatTGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTAtcagcgtcagc < 1:786552/81‑1 (MQ=255)
ttGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAgcgc < 2:786553/81‑1 (MQ=255)
ttGATGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAgcgc > 2:786589/1‑81 (MQ=255)
tGGCATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATc > 1:786600/1‑81 (MQ=255)
|
TCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATC > NC_000913/4293521‑4293675
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
