Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,319,653 |
G→A |
F337F (TTC→TTT) |
phnI ← |
carbon‑phosphorus lyase core complex subunit PhnI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,319,653 | 0 | G | A | 100.0%
| 132.9
/ NA
| 38 | F337F (TTC→TTT) | phnI | carbon‑phosphorus lyase core complex subunit PhnI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/25); total (13/25) |
GCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGGAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACG > NC_000913/4319578‑4319728
|
gCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATc < 2:792264/81‑1 (MQ=255)
aaGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGAc < 1:792266/81‑1 (MQ=255)
aaGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGAc < 1:792265/81‑1 (MQ=255)
ttGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACg > 1:792318/1‑79 (MQ=255)
tGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAg > 2:792327/1‑81 (MQ=255)
aGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTgg > 2:792334/1‑81 (MQ=255)
ccGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTgggg < 2:792267/81‑1 (MQ=255)
cGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTggggg < 2:792268/81‑1 (MQ=255)
aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGttt < 1:792269/81‑1 (MQ=255)
aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGtt < 1:792270/80‑1 (MQ=255)
gATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTGGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTg < 1:792271/81‑1 (MQ=255)
aTTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGa < 2:792272/81‑1 (MQ=255)
gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtg > 2:792337/1‑81 (MQ=255)
tGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtgt > 2:792326/1‑78 (MQ=255)
tGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTgag < 2:792273/81‑1 (MQ=255)
cTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACaa < 1:792274/80‑1 (MQ=255)
gCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGc > 2:792309/1‑81 (MQ=255)
cTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGc > 2:792333/1‑81 (MQ=255)
ttGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGc < 2:792275/81‑1 (MQ=255)
gCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCt < 2:792276/80‑1 (MQ=255)
cAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTc < 2:792277/81‑1 (MQ=255)
aCGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGAc < 1:792278/81‑1 (MQ=255)
gTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGt > 2:792348/1‑81 (MQ=255)
tttGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGGAGTGTGAAACAAAGCCTGCGGCTTCGACGtt < 2:792280/81‑1 (MQ=255)
tttGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGtt < 2:792279/81‑1 (MQ=255)
aGTAGCTCCAGTTCGGCCTGAAAATCGACGTGGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCg < 2:792283/81‑1 (MQ=255)
aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTc < 2:792284/80‑1 (MQ=255)
aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTc < 1:792282/80‑1 (MQ=255)
aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCg < 1:792281/81‑1 (MQ=255)
aGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCa > 2:792335/1‑81 (MQ=255)
ccAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgtg > 2:792344/1‑81 (MQ=255)
ccAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgtg > 1:792341/1‑81 (MQ=255)
cAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgt < 1:792285/79‑1 (MQ=255)
gTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGc > 1:792336/1‑79 (MQ=255)
cGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGc < 2:792287/80‑1 (MQ=255)
cGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGc < 1:792286/80‑1 (MQ=255)
gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCAc > 2:792358/1‑80 (MQ=255)
gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACg < 1:792288/81‑1 (MQ=255)
|
GCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGGAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACG > NC_000913/4319578‑4319728
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
