Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,323,424 |
G→T |
T310N (ACC→AAC) |
phnD ← |
phosphonate ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,323,424 | 0 | G | T | 100.0%
| 113.2
/ NA
| 31 | T310N (ACC→AAC) | phnD | phosphonate ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/18); total (13/18) |
TTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGGTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCGCGAG > NC_000913/4323348‑4323497
|
tttACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTtttt > 2:863836/1‑81 (MQ=255)
aCTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTg < 2:863780/80‑1 (MQ=255)
cTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGc < 1:863781/80‑1 (MQ=255)
cGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCtt < 2:863782/81‑1 (MQ=255)
aaCTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGt < 1:863783/81‑1 (MQ=255)
tCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCt > 1:863833/1‑81 (MQ=255)
aTCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCt < 1:863784/79‑1 (MQ=255)
cgcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAt < 2:863785/81‑1 (MQ=255)
gcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAtt < 2:863786/81‑1 (MQ=255)
gcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAtt > 1:863825/1‑81 (MQ=255)
aGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCa < 1:863787/79‑1 (MQ=255)
gcgTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCt > 1:863834/1‑81 (MQ=255)
gcgTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCt > 1:863843/1‑81 (MQ=255)
gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCttt < 1:863788/81‑1 (MQ=255)
gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCtt > 1:863824/1‑80 (MQ=255)
gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCtt > 1:863829/1‑80 (MQ=255)
gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCtt > 1:863832/1‑80 (MQ=255)
aGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCtt < 2:863789/81‑1 (MQ=255)
gCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCtttt > 1:863835/1‑81 (MQ=255)
cGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTc < 2:863790/81‑1 (MQ=255)
aGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGccc > 1:863838/1‑81 (MQ=255)
aGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGccc > 2:863837/1‑81 (MQ=255)
cATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCCTTTCACGCCCTGCa > 1:863840/1‑81 (MQ=255)
tCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCAtc < 2:863791/81‑1 (MQ=255)
cAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCAtct < 1:863792/80‑1 (MQ=255)
aGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATctct < 1:863793/81‑1 (MQ=255)
cTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCttt < 1:863794/81‑1 (MQ=255)
gcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTaaa > 2:863847/1‑80 (MQ=255)
aaTCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAgcgc < 2:863796/81‑1 (MQ=255)
aaTCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAgc < 1:863795/79‑1 (MQ=255)
aTCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGcgcg < 1:863797/81‑1 (MQ=255)
ggtgttTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCGCGAg < 2:863798/78‑1 (MQ=255)
|
TTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGGTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCGCGAG > NC_000913/4323348‑4323497
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
