Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,323,424 |
G→T |
T310N (ACC→AAC) |
phnD ← |
phosphonate ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,323,424 | 0 | G | T | 100.0%
| 116.7
/ NA
| 34 | T310N (ACC→AAC) | phnD | phosphonate ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/18); total (16/18) |
GCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGGTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCG > NC_000913/4323346‑4323493
|
gCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGttt > 1:800584/1‑81 (MQ=255)
cTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGtttt > 2:793431/1‑81 (MQ=255)
tCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGc > 2:793407/1‑79 (MQ=255)
cACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAg > 1:793410/1‑81 (MQ=255)
ccGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCt < 1:793376/81‑1 (MQ=255)
ccGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCt < 1:793375/81‑1 (MQ=255)
cGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCtt < 2:793377/81‑1 (MQ=255)
gAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTg > 2:793378/1‑81 (MQ=255)
aaCTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGt > 1:793424/1‑81 (MQ=255)
tCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCt > 2:793417/1‑81 (MQ=255)
aTCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCt < 1:793379/79‑1 (MQ=255)
tCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCt < 2:793380/81‑1 (MQ=255)
cgcgCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCt > 1:793423/1‑80 (MQ=255)
gcgcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCa < 2:793381/81‑1 (MQ=255)
gcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAtt > 2:793419/1‑81 (MQ=255)
gcgcGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTc > 1:793418/1‑81 (MQ=255)
cgcgTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTcc < 1:793382/81‑1 (MQ=255)
gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCttt < 2:793383/81‑1 (MQ=255)
ttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTg > 2:793422/1‑81 (MQ=255)
cAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCt > 2:793416/1‑81 (MQ=255)
aGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCtt < 2:793384/81‑1 (MQ=255)
aGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCtt < 1:793385/81‑1 (MQ=255)
ccAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGc > 1:793421/1‑81 (MQ=255)
tCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGc < 2:793386/81‑1 (MQ=255)
cAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCAtct < 1:793387/80‑1 (MQ=255)
cTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCttt < 2:793388/81‑1 (MQ=255)
cTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCttt < 2:793389/81‑1 (MQ=255)
cTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCttt < 2:793390/81‑1 (MQ=255)
cgcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTaaa > 2:793429/1‑81 (MQ=255)
cgcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTaaa > 1:793426/1‑81 (MQ=255)
cgcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTa < 2:793391/79‑1 (MQ=255)
gcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTaaa > 1:793434/1‑80 (MQ=255)
gcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAAc < 1:793392/81‑1 (MQ=255)
cTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAg < 1:793393/81‑1 (MQ=255)
cTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAg > 1:793433/1‑81 (MQ=255)
gAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAgcg > 1:793428/1‑81 (MQ=255)
|
GCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGGTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCG > NC_000913/4323346‑4323493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
