Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,334,130 |
C→T |
W487* (TGG→TGA) |
eptA ← |
phosphoethanolamine transferase EptA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,334,130 | 0 | C | T | 100.0%
| 67.9
/ NA
| 20 | W487* (TGG→TGA) | eptA | phosphoethanolamine transferase EptA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/15); total (5/15) |
AATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAT > NC_000913/4334054‑4334203
|
aaTAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTcac < 1:795628/80‑1 (MQ=255)
tAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCAcagc < 1:795629/81‑1 (MQ=255)
tgtTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACagcagc < 2:795630/81‑1 (MQ=255)
gtTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAgcagca > 1:795635/1‑81 (MQ=255)
ttGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAgcagca < 1:795631/80‑1 (MQ=255)
gTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGc < 2:795632/81‑1 (MQ=255)
cgcCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCt < 2:795633/80‑1 (MQ=255)
tttttGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGtttt < 2:795634/79‑1 (MQ=255)
tttGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTgg < 2:795635/80‑1 (MQ=255)
caggcagTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATc < 2:795636/81‑1 (MQ=255)
aggcagTTCTGGTCAACCTGATACCATTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTGGCCTATcc > 2:795655/1‑81 (MQ=255)
ggcagTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCg < 1:795637/81‑1 (MQ=255)
agTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCgggg > 1:795650/1‑81 (MQ=255)
gTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGc < 1:795639/81‑1 (MQ=255)
gTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGc < 2:795638/81‑1 (MQ=255)
aTACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAg < 2:795640/81‑1 (MQ=255)
gTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGt < 1:795641/81‑1 (MQ=255)
ctccGACAGTCACAGCAGCATCTGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGcc < 2:795642/81‑1 (MQ=255)
ccGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAt > 1:795659/1‑81 (MQ=255)
ccGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAt > 2:795660/1‑81 (MQ=255)
|
AATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAT > NC_000913/4334054‑4334203
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
