Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,346,977 |
T→A |
K117I (AAA→ATA) |
fumB ← |
fumarase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,346,977 | 0 | T | A | 100.0%
| 104.2
/ NA
| 30 | K117I (AAA→ATA) | fumB | fumarase B |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (13/17); total (13/17) |
ATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTT > NC_000913/4346901‑4347054
|
aTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtattt > 2:798160/1‑80 (MQ=255)
aTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtattt < 2:798110/80‑1 (MQ=255)
tCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtatttt < 2:798111/80‑1 (MQ=255)
tCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTAcc > 2:798157/1‑80 (MQ=255)
cGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACcga > 1:798168/1‑81 (MQ=255)
ggTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACgatgat < 2:798114/81‑1 (MQ=255)
ggTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACgatgat < 2:798113/81‑1 (MQ=255)
ggTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACgatgat < 2:798112/81‑1 (MQ=255)
gtgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATc < 2:798115/81‑1 (MQ=255)
tgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATcg < 1:798116/81‑1 (MQ=255)
gtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATcgc > 2:798165/1‑81 (MQ=255)
ttATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATcgc < 1:798117/80‑1 (MQ=255)
taGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGt < 1:798118/80‑1 (MQ=255)
cGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCgg < 1:798119/81‑1 (MQ=255)
cTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTAt > 2:798159/1‑81 (MQ=255)
tttCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTTCCGGTATc > 2:798175/1‑81 (MQ=255)
tttCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATc > 1:798162/1‑81 (MQ=255)
tCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCt > 2:798178/1‑81 (MQ=255)
gACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTgg > 2:798163/1‑81 (MQ=255)
gCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAgg < 2:798120/81‑1 (MQ=255)
gTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTc < 1:798121/81‑1 (MQ=255)
cttcATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCgg > 1:798172/1‑79 (MQ=255)
cttcATCACCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGgc < 1:798122/80‑1 (MQ=255)
atcaCCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCAc > 2:798170/1‑81 (MQ=255)
caCCGCCGCCGGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGc > 1:798167/1‑81 (MQ=255)
cgccgccgGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCtt < 1:798123/81‑1 (MQ=255)
gccgccgGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCttt < 1:798124/81‑1 (MQ=255)
cgccgGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTgg > 1:798148/1‑81 (MQ=255)
gccgGTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggc > 2:798176/1‑81 (MQ=255)
ggTCCACACGCGCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcggc < 2:798125/81‑1 (MQ=255)
cgcgCTGGCCTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCgg < 1:798126/81‑1 (MQ=255)
ccTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCtt < 1:798127/81‑1 (MQ=255)
ccTATTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCtt < 1:798128/81‑1 (MQ=255)
|
ATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTT > NC_000913/4346901‑4347054
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
