Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,431,837 |
C→A |
E49D (GAG→GAT) |
ytfE ← |
iron‑sulfur cluster repair protein YtfE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,431,837 | 0 | C | A | 100.0%
| 103.2
/ NA
| 30 | E49D (GAG→GAT) | ytfE | iron‑sulfur cluster repair protein YtfE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/17); total (13/17) |
ATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATC > NC_000913/4431760‑4431908
|
atTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATc > 2:815778/1‑80 (MQ=255)
ccAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCa < 1:815740/81‑1 (MQ=255)
cAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAg > 2:815774/1‑81 (MQ=255)
ggggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCt < 2:815741/81‑1 (MQ=255)
cgcTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTAc < 1:815742/81‑1 (MQ=255)
cgcTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTAc < 2:815743/81‑1 (MQ=255)
aCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgc > 1:815768/1‑81 (MQ=255)
cGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgc < 2:815744/80‑1 (MQ=255)
gCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgccg > 1:815784/1‑81 (MQ=255)
ccAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgccgc < 1:815745/81‑1 (MQ=255)
cAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTgccgcc < 2:815746/81‑1 (MQ=255)
tctcAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCcgcgc < 2:815747/78‑1 (MQ=255)
aaTCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCa < 2:815748/80‑1 (MQ=255)
aTCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGc > 2:815767/1‑81 (MQ=255)
ggTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTc > 2:815785/1‑81 (MQ=255)
gttgttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCt > 2:815783/1‑81 (MQ=255)
gttgttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCt < 1:815749/81‑1 (MQ=255)
tCAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTa > 1:815789/1‑81 (MQ=255)
cAGCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTa < 2:815750/80‑1 (MQ=255)
gCGAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCg < 1:815751/81‑1 (MQ=255)
gAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGc > 1:815819/1‑80 (MQ=255)
gAGCTTTGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGc > 2:815799/1‑80 (MQ=255)
ttGCCAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcag > 2:815790/1‑80 (MQ=255)
ccAGTTCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag < 1:815752/80‑1 (MQ=255)
tCAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATc > 2:815791/1‑80 (MQ=255)
cAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc < 2:815753/80‑1 (MQ=255)
cAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc < 2:815754/80‑1 (MQ=255)
cAGCTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc < 1:815755/80‑1 (MQ=255)
cTTCAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCcatat > 2:815795/1‑81 (MQ=255)
cAATGACATCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCcatatc < 2:815756/79‑1 (MQ=255)
|
ATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATC > NC_000913/4431760‑4431908
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
