Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,434,404 |
C→T |
Q98* (CAG→TAG) |
ytfH → |
putative transcriptional regulator YtfH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,434,404 | 0 | C | T | 100.0%
| 134.6
/ NA
| 38 | Q98* (CAG→TAG) | ytfH | putative transcriptional regulator YtfH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/21); total (17/21) |
CAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGG > NC_000913/4434326‑4434475
|
cAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAg < 1:816278/81‑1 (MQ=255)
aGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAgg > 1:816328/1‑81 (MQ=255)
gATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTg < 1:816279/81‑1 (MQ=255)
aTGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGa < 2:816280/81‑1 (MQ=255)
tGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAg < 2:816281/81‑1 (MQ=255)
gTTTTCTTAACCGTATCGCGTATCCGGTGGAGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGc < 2:816282/80‑1 (MQ=255)
tCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGaaa < 2:816283/80‑1 (MQ=255)
cTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGaaaa < 1:816284/80‑1 (MQ=255)
cTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAg > 2:816332/1‑81 (MQ=255)
cTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAg > 2:816318/1‑81 (MQ=255)
tAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGt > 2:816336/1‑81 (MQ=255)
tAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGt > 2:816330/1‑81 (MQ=255)
aaCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGtt > 1:816320/1‑81 (MQ=255)
aaCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGt < 2:816285/80‑1 (MQ=255)
aCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGtt < 2:816286/80‑1 (MQ=255)
tCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCa < 1:816287/80‑1 (MQ=255)
cgcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACt > 1:816337/1‑81 (MQ=255)
gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACt < 1:816288/80‑1 (MQ=255)
gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTg > 1:816329/1‑81 (MQ=255)
gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTg > 1:816323/1‑81 (MQ=255)
gTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGc > 1:816341/1‑81 (MQ=255)
tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCg < 2:816289/81‑1 (MQ=255)
cGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACt < 1:816290/81‑1 (MQ=255)
ggtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGAt > 2:816344/1‑81 (MQ=255)
gccgccGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGAtt > 2:816350/1‑79 (MQ=255)
ccgccgCATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAg > 2:816343/1‑81 (MQ=255)
gccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGtt < 2:816291/81‑1 (MQ=255)
aTGTGGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAAtt > 2:816345/1‑81 (MQ=255)
tgGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGGGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGc < 1:816292/81‑1 (MQ=255)
tgGAATATAGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGc < 1:816293/81‑1 (MQ=255)
tataGCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAg < 1:816294/81‑1 (MQ=255)
gCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGgtgt > 2:816342/1‑81 (MQ=255)
gCCTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGgtg < 1:816295/80‑1 (MQ=255)
ccTCACGCCGCTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGtt < 1:816296/81‑1 (MQ=255)
cgcTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGc > 1:816356/1‑81 (MQ=255)
cgcTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGc < 2:816297/81‑1 (MQ=255)
gcTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCg < 1:816298/81‑1 (MQ=255)
cTGGGCGAGTAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCgg > 2:816347/1‑81 (MQ=255)
|
CAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGG > NC_000913/4434326‑4434475
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
