Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,511,496 |
C→A |
A307S (GCG→TCG) |
fecD ← |
ferric citrate ABC transporter membrane subunit FecD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,511,496 | 0 | C | A | 100.0%
| 108.7
/ NA
| 31 | A307S (GCG→TCG) | fecD | ferric citrate ABC transporter membrane subunit FecD |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/15); total (16/15) |
CCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCA > NC_000913/4511419‑4511573
|
cccGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAAcc > 2:831321/1‑81 (MQ=255)
cGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGa > 2:831330/1‑81 (MQ=255)
cGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGa < 2:831291/81‑1 (MQ=255)
gTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGa > 2:831334/1‑80 (MQ=255)
aaCTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATaa > 2:831328/1‑81 (MQ=255)
aaCTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATaa > 1:831325/1‑81 (MQ=255)
aCTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAAt < 1:831292/81‑1 (MQ=255)
gTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTc < 2:831293/81‑1 (MQ=255)
aGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAg > 2:831342/1‑80 (MQ=255)
aGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGc < 2:831294/81‑1 (MQ=255)
gATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCa < 2:831295/81‑1 (MQ=255)
tCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGcc > 2:831331/1‑80 (MQ=255)
aGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCaa < 2:831296/80‑1 (MQ=255)
gTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAAcc < 1:831297/81‑1 (MQ=255)
gTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAAcc < 2:831298/81‑1 (MQ=255)
ttCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCg < 1:831299/81‑1 (MQ=255)
gTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGa < 1:831300/81‑1 (MQ=255)
aaGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGc < 1:831301/80‑1 (MQ=255)
aaGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCt > 2:831344/1‑81 (MQ=255)
aGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTc < 1:831302/81‑1 (MQ=255)
aTTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGt > 1:831348/1‑81 (MQ=255)
tATCGCATTCTCACAAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTgg < 2:831303/80‑1 (MQ=255)
acaAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCAAGTGGGGGATGAATAAtt > 2:831308/1‑81 (MQ=255)
caAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtc > 1:831335/1‑81 (MQ=255)
caAGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtc > 2:831333/1‑81 (MQ=255)
aGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtctc < 1:831304/81‑1 (MQ=255)
aGCAACCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATtctc > 1:831350/1‑81 (MQ=255)
aaCCAGACAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGcc < 1:831305/81‑1 (MQ=255)
cAAACCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCcag > 1:831343/1‑76 (MQ=255)
aaaCCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCcag > 2:831312/1‑75 (MQ=255)
aaCCACGGCGAACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCcag > 1:831347/1‑74 (MQ=255)
gcgaACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAacca > 1:831337/1‑81 (MQ=255)
|
CCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCCAGTGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCA > NC_000913/4511419‑4511573
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
