Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,551,712 |
G→A |
G26D (GGC→GAC) |
uxuA → |
D‑mannonate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,551,712 | 0 | G | A | 100.0%
| 120.3
/ NA
| 34 | G26D (GGC→GAC) | uxuA | D‑mannonate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/10); total (24/10) |
TGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACG > NC_000913/4551637‑4551778
|
tGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTgg > 1:839011/1‑81 (MQ=255)
gAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGt > 2:838999/1‑80 (MQ=255)
gAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGt > 1:838987/1‑80 (MQ=255)
gAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGt < 2:838956/80‑1 (MQ=255)
aCAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTAc > 1:838985/1‑81 (MQ=255)
cAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTAcc > 2:839003/1‑81 (MQ=255)
gACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACcgc > 1:838991/1‑81 (MQ=255)
ccTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCgcgc > 2:838990/1‑81 (MQ=255)
cTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCt < 1:838957/81‑1 (MQ=255)
tGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTg > 2:838995/1‑81 (MQ=255)
gcgcTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGc < 1:838958/80‑1 (MQ=255)
cTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCa > 1:838992/1‑81 (MQ=255)
cTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCa < 2:838959/81‑1 (MQ=255)
ggTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCata > 1:839007/1‑81 (MQ=255)
gTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCatat > 2:838996/1‑81 (MQ=255)
tACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATc > 2:838989/1‑81 (MQ=255)
cGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATccc < 1:838960/81‑1 (MQ=255)
ggCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATccc > 1:839002/1‑80 (MQ=255)
aaaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACgg > 1:839010/1‑81 (MQ=255)
aaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGc > 2:839000/1‑81 (MQ=255)
ccGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTa > 1:839012/1‑81 (MQ=255)
cGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTAt < 1:838961/81‑1 (MQ=255)
ggTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATg > 1:839006/1‑81 (MQ=255)
gTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATgg > 2:839001/1‑81 (MQ=255)
tttCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGt > 1:839031/1‑81 (MQ=255)
cTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTcc < 1:838962/80‑1 (MQ=255)
tAGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGt < 2:838963/79‑1 (MQ=255)
aGCTGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTg < 2:838964/80‑1 (MQ=255)
tGATGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGaa > 1:839009/1‑79 (MQ=255)
aTGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGaa > 2:839013/1‑77 (MQ=255)
aTGTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAagag < 2:838965/80‑1 (MQ=255)
gTCCGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATc > 2:839020/1‑81 (MQ=255)
ccGTCAGGCGGGCGCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCt > 1:839021/1‑81 (MQ=255)
gcgggcgCAACTGACGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACg > 2:839005/1‑80 (MQ=255)
|
TGGAACAGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACG > NC_000913/4551637‑4551778
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
