Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,183,748 |
A→G |
E835G (GAA→GGA) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,183,748 | 0 | A | G | 100.0%
| 163.4
/ NA
| 44 | E835G (GAA→GGA) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/20); total (24/20) |
ACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTG > NC_000913/4183673‑4183825
|
aCAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTg < 1:829862/80‑1 (MQ=255)
ttCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgt > 1:829910/1‑81 (MQ=255)
cGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgtgt > 1:829918/1‑81 (MQ=255)
cGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgtgt > 2:829931/1‑81 (MQ=255)
gAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgtgtg > 1:829913/1‑81 (MQ=255)
aCTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTccc < 2:829863/81‑1 (MQ=255)
aCTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTccc > 2:829933/1‑81 (MQ=255)
cATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGt < 2:829864/79‑1 (MQ=255)
cATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGa > 1:829950/1‑81 (MQ=255)
gTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAg > 2:829934/1‑81 (MQ=255)
gTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAg > 2:829944/1‑81 (MQ=255)
tATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGc < 1:829865/81‑1 (MQ=255)
tATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGc > 1:829920/1‑81 (MQ=255)
cGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTggg < 2:829866/81‑1 (MQ=255)
gAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGt > 1:829935/1‑81 (MQ=255)
gCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTcc > 2:829921/1‑81 (MQ=255)
tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGa < 1:829867/81‑1 (MQ=255)
tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGa < 1:829868/81‑1 (MQ=255)
tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGa < 1:829869/81‑1 (MQ=255)
gttgttCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCgg > 1:829938/1‑79 (MQ=255)
gttCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAaga < 1:829870/80‑1 (MQ=255)
ttCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAagag < 1:829871/80‑1 (MQ=255)
tCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGaa > 2:829927/1‑76 (MQ=255)
aGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATc < 2:829873/80‑1 (MQ=255)
aGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCa < 2:829872/81‑1 (MQ=255)
aGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCa > 1:829942/1‑81 (MQ=255)
ggAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCAc > 1:829941/1‑81 (MQ=255)
gAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCAcc > 2:829946/1‑81 (MQ=255)
aGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGc > 2:829926/1‑81 (MQ=255)
aGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGc < 1:829874/81‑1 (MQ=255)
cGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGAc < 2:829875/81‑1 (MQ=255)
cGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGAc < 1:829876/81‑1 (MQ=255)
tCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATcc > 2:829930/1‑81 (MQ=255)
caccacCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATcc < 1:829877/80‑1 (MQ=255)
accaccATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATccc > 1:829948/1‑80 (MQ=255)
tCCACATTCTGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTgg > 2:829945/1‑81 (MQ=255)
cacaTTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTggg < 1:829878/80‑1 (MQ=255)
ttCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAg < 1:829879/81‑1 (MQ=255)
tCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGc > 1:829912/1‑81 (MQ=255)
cAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAgat > 2:829947/1‑79 (MQ=255)
cAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGc < 1:829880/80‑1 (MQ=255)
aGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCt < 2:829881/80‑1 (MQ=255)
aGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTg > 2:829929/1‑81 (MQ=255)
aGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTg > 1:829936/1‑81 (MQ=255)
|
ACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTG > NC_000913/4183673‑4183825
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
