Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,418,094 |
G→A |
I120I (ATC→ATT) |
ulaR ← |
DNA‑binding transcriptional repressor UlaR |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,418,094 | 0 | G | A | 100.0%
| 88.2
/ NA
| 25 | I120I (ATC→ATT) | ulaR | DNA‑binding transcriptional repressor UlaR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/10); total (15/10) |
TTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCTT > NC_000913/4418017‑4418169
|
ttgtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaat < 1:885618/80‑1 (MQ=255)
tgtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaatga > 2:885658/1‑81 (MQ=255)
gtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaatga < 1:885619/80‑1 (MQ=255)
gtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaatga < 2:885620/80‑1 (MQ=255)
cTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTg < 2:885621/81‑1 (MQ=255)
gTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCa > 1:885659/1‑81 (MQ=255)
tCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCAc < 2:885622/81‑1 (MQ=255)
ccGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACt > 2:885671/1‑81 (MQ=255)
cccATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGc < 2:885623/81‑1 (MQ=255)
tAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGc > 2:885665/1‑81 (MQ=255)
aaTGATCACGCTGTCATGTTCTTGGTCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATTATTTGCACTGGCTTGc > 1:885668/1‑80 (MQ=255)
tGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCcac < 2:885624/81‑1 (MQ=255)
aTCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCcaca > 1:885666/1‑80 (MQ=255)
cTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTcc < 2:885625/81‑1 (MQ=255)
gTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCg > 2:885663/1‑81 (MQ=255)
gatcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGa > 1:885656/1‑80 (MQ=255)
gatcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGa > 2:885664/1‑80 (MQ=255)
atcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGaa < 1:885626/80‑1 (MQ=255)
tcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAAc < 2:885627/81‑1 (MQ=255)
tcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCgg < 1:885628/81‑1 (MQ=255)
ggTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTg > 1:885670/1‑80 (MQ=255)
gTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGa < 1:885629/81‑1 (MQ=255)
cAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGtt > 1:885662/1‑81 (MQ=255)
aGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGtt > 2:885675/1‑80 (MQ=255)
ggTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTtgat > 1:885674/1‑80 (MQ=255)
aTAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTAc > 2:885669/1‑81 (MQ=255)
aTAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTAc > 2:885678/1‑81 (MQ=255)
aTTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCt > 2:885673/1‑81 (MQ=255)
ttAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCtt < 2:885630/81‑1 (MQ=255)
|
TTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCTT > NC_000913/4418017‑4418169
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
