Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,480,873 |
G→A |
G382S (GGC→AGC) |
yjgN → |
conserved inner membrane protein YjgN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,480,873 | 0 | G | A | 100.0%
| 105.3
/ NA
| 29 | G382S (GGC→AGC) | yjgN | conserved inner membrane protein YjgN |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/9); total (20/9) |
CAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGC > NC_000913/4480802‑4480950
|
caAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAg < 1:899983/81‑1 (MQ=255)
cGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTAttt < 2:899985/81‑1 (MQ=255)
cGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTAtt < 1:899984/80‑1 (MQ=255)
gTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTAttt > 1:900003/1‑80 (MQ=255)
tGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACggg > 2:900014/1‑81 (MQ=255)
gCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACggg > 2:900020/1‑80 (MQ=255)
tGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAg > 2:900016/1‑81 (MQ=255)
ggCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGtt < 1:899986/81‑1 (MQ=255)
ggCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGt < 1:899987/80‑1 (MQ=255)
gCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTa > 1:900027/1‑81 (MQ=255)
gCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTa > 2:900011/1‑81 (MQ=255)
gCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTa > 2:900005/1‑81 (MQ=255)
tGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCctt > 1:900013/1‑81 (MQ=255)
ggATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCcttc > 1:900031/1‑81 (MQ=255)
cttccttTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCa > 2:900021/1‑81 (MQ=255)
tccttTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCAtt > 1:900028/1‑81 (MQ=255)
tAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCt < 2:899988/79‑1 (MQ=255)
aTCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGt > 1:900026/1‑79 (MQ=255)
tCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGt > 1:900015/1‑78 (MQ=255)
tAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt < 2:899989/81‑1 (MQ=255)
aaaGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt > 2:900025/1‑80 (MQ=255)
aaaGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt > 2:900009/1‑80 (MQ=255)
aaaGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt > 1:900012/1‑80 (MQ=255)
agaagaACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaa < 1:899990/81‑1 (MQ=255)
gaagaaCAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa < 1:899991/81‑1 (MQ=255)
agaaCAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa > 1:900017/1‑79 (MQ=255)
aacaacCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAgg > 1:900023/1‑81 (MQ=255)
cAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAg > 2:900033/1‑81 (MQ=255)
gATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCa > 1:900019/1‑81 (MQ=255)
aaaaGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGc > 1:900024/1‑81 (MQ=255)
|
CAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGC > NC_000913/4480802‑4480950
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
