Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F130 I1 R1 38 76.4 2725422 95.3% 2597327 137.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,397,330 G→T M1M (ATG→ATT) † mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,397,3300GT98.6% 241.9 / NA 70M1M (ATG→ATT) †mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (37/31);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTG  >  NC_000913_3_pae_tpiA/4397184‑4397475
                                                                                                                                                  |                                                                                                                                                 
caacaGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCa                                                                                                                                                >  1:20091/1‑150 (MQ=255)
caacaGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCa                                                                                                                                                >  2:86418/1‑150 (MQ=255)
     gCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCa                                                                                                                                           >  3:262949/1‑150 (MQ=255)
      cTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAg                                                                                                                                          <  4:206102/150‑1 (MQ=255)
          cAGAAGCCATTTACAAAGGCCTGCGCAATTACTGCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCt                                                                                                                                      <  2:812027/150‑1 (MQ=255)
             aaGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTAc                                                                                                                                   >  2:378693/1‑150 (MQ=255)
                 cATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGcc                                                                                                                               <  1:95741/150‑1 (MQ=255)
                        aaaGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTg                                                                                                                        >  4:160850/1‑150 (MQ=255)
                        aaaGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTg                                                                                                                        <  3:327695/150‑1 (MQ=255)
                         aaGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTgg                                                                                                                       <  4:86112/150‑1 (MQ=255)
                               tGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACACCCTACCAGTACGGTGACGACGCCAGATCGCACGATGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAAcc                                                                                                                 >  1:398550/1‑150 (MQ=255)
                               tGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGCTTGATTCCAATTCAGGTCTTACCGCCACAACTGGAGAAcc                                                                                                                 >  3:83044/1‑150 (MQ=255)
                                gcgcAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCa                                                                                                                >  4:50215/1‑150 (MQ=255)
                                    aaTTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGAtt                                                                                                            >  1:763570/1‑150 (MQ=255)
                                         cTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGATGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGc                                                                                                       <  2:672710/150‑1 (MQ=255)
                                         cTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTgg                                                                                                                       >  3:138877/1‑134 (MQ=255)
                                         cTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTgg                                                                                                                       <  4:138877/134‑1 (MQ=255)
                                          ttCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCa                                                                                                      >  4:349735/1‑150 (MQ=255)
                                            ccTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCagg                                                                                                    >  4:297892/1‑150 (MQ=255)
                                             cTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggt                                                                                                   <  2:763570/150‑1 (MQ=255)
                                              ttGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggtg                                                                                                  >  1:499415/1‑150 (MQ=255)
                                                gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggtgag                                                                                                >  1:654128/1‑150 (MQ=255)
                                                gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggtgag                                                                                                >  1:38955/1‑150 (MQ=255)
                                                  gcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggtgaggt                                                                                              >  1:364312/1‑150 (MQ=255)
                                                  gcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggtgaggt                                                                                              <  1:687079/150‑1 (MQ=255)
                                                   cATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCaggtgaggtg                                                                                             >  3:10286/1‑150 (MQ=255)
                                                     tCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAggtggt                                                                                           <  1:325728/150‑1 (MQ=255)
                                                      ccGATGCAATCTGCTCCTCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTc                                                                                          <  1:647757/150‑1 (MQ=255)
                                                        gATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGa                                                                                        <  4:189243/150‑1 (MQ=255)
                                                             aaTCTGCGCCGCCGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGAc                                                                                   <  1:362895/150‑1 (MQ=255)
                                                               tCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCt                                                                                 >  1:355201/1‑150 (MQ=255)
                                                                 tGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGc                                                                               >  3:387325/1‑150 (MQ=255)
                                                                     ccgcAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCg                                                                           <  3:518292/150‑1 (MQ=255)
                                                                        cAGGGTGCAACGGCACCAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACACCTGGCGAACCAGGTTGCCGCAGGTGATGTGGTCGAGCGACCTGCGTCGGTa                                                                        <  2:716959/150‑1 (MQ=255)
                                                                             tGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaa                                                                   >  4:284612/1‑150 (MQ=255)
                                                                              gCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaaa                                                                  <  1:642487/150‑1 (MQ=255)
                                                                              gCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaaa                                                                  <  1:642484/150‑1 (MQ=255)
                                                                                    gCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTa                                                            <  3:72281/150‑1 (MQ=255)
                                                                                     cacaAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAg                                                           >  2:527666/1‑150 (MQ=255)
                                                                                       caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTg                                                         >  2:611351/1‑150 (MQ=255)
                                                                                        aaaCTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTgg                                                        >  3:13417/1‑150 (MQ=255)
                                                                                        aaaCTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTgg                                                        >  2:768759/1‑150 (MQ=255)
                                                                                        aaaCTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTgg                                                        >  3:406967/1‑150 (MQ=255)
                                                                                          aCTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGGGGGGTGAGTCCAATTCAGGGCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGaa                                                      <  1:331271/150‑1 (MQ=255)
                                                                                               cAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAg                                                 >  2:304257/1‑150 (MQ=255)
                                                                                                 atACGGATACGACGACAGATAGGACTCTGCCAAACAAAGGGCGATTGATACCAAGTCAGGTCTTACAGCAACGACTGGCGAACCAGATTGGCGCAGGTGAGGTGGGCGAGCGACCTGCGTCGGTAGGCAAAGAGCTTGTGGAAAACGGcc                                               <  2:186972/149‑1 (MQ=255)
                                                                                                     ggTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGa                                           <  1:199020/150‑1 (MQ=255)
                                                                                                      gTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGAt                                          <  3:470206/150‑1 (MQ=255)
                                                                                                      gTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGAt                                          <  3:50215/150‑1 (MQ=255)
                                                                                                        gacgacGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGc                                        <  4:309003/150‑1 (MQ=255)
                                                                                                         acgactCCAGATCGCACGCTTCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCGTCTTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCa                                       <  4:127441/150‑1 (MQ=255)
                                                                                                         acgacgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCa                                       <  1:588833/150‑1 (MQ=255)
                                                                                                           gacgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAgg                                     >  3:221860/1‑150 (MQ=255)
                                                                                                            acgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACACCTGGCGAACCAGATTGCCGCGGTTGAGGCGGGCGAGCGACCTGCGGCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGt                                    >  3:21319/1‑150 (MQ=255)
                                                                                                            acgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGGCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGt                                    >  3:21325/1‑150 (MQ=255)
                                                                                                              gCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTg                                                                                                                        <  2:67131/64‑1 (MQ=255)
                                                                                                              gCCAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTg                                                                                                                        >  1:67131/1‑64 (MQ=255)
                                                                                                                cAGATCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTTGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGa                                >  2:141124/1‑150 (MQ=255)
                                                                                                                   aTCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcgc                             <  2:38955/150‑1 (MQ=255)
                                                                                                                   aTCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcgc                             >  4:72458/1‑150 (MQ=255)
                                                                                                                    tCGCACGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcgcg                            >  4:262369/1‑150 (MQ=255)
                                                                                                                         cGCTGCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCg                       >  4:412533/1‑150 (MQ=255)
                                                                                                                             gCCAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                   >  3:497638/1‑150 (MQ=255)
                                                                                                                              ccAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATAtt                  <  2:570593/150‑1 (MQ=255)
                                                                                                                               cAAACTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACCAGTGGAAAACAGCCTCGATGCAGGCGCGACGCGTATCGATATTg                 >  1:541543/1‑150 (MQ=255)
                                                                                                                                   cTAAGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGatat             <  3:349735/150‑1 (MQ=255)
                                                                                                                                     aaGGACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCg           >  1:308191/1‑150 (MQ=255)
                                                                                                                                       ggACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGaa         <  2:499415/150‑1 (MQ=255)
                                                                                                                                       ggACGATTGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGaa         <  3:262369/150‑1 (MQ=255)
                                                                                                                                              tGATTCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTg  <  1:551212/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTG  >  NC_000913_3_pae_tpiA/4397184‑4397475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A