Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F19 I1 R1
|
15 |
12.7 |
481992 |
93.3% |
449698 |
131.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_pae_tpiA |
4,367,616 |
3 bp→TCC |
coding (626‑628/1437 nt) |
aspA ← |
aspartate ammonia‑lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_pae_tpiA | 4,367,616 | 0 | G | T | 100.0%
| 12.6
/ NA
| 5 | L210M (CTG→ATG) | aspA | aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (2/3); total (2/3) |
| * | NC_000913_3_pae_tpiA | 4,367,617 | 0 | G | C | 100.0%
| 12.1
/ NA
| 5 | I209R (ATC→AGG) | aspA | aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (2/3); total (2/3) |
| * | NC_000913_3_pae_tpiA | 4,367,618 | 0 | A | C | 100.0%
| 12.4
/ NA
| 5 | I209R (ATC→AGG) | aspA | aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (2/3); total (2/3) |
CAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGC > NC_000913_3_pae_tpiA/4367481‑4367630
|||
cAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaag < 1:48039/150‑4 (MQ=255)
cTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAga > 1:123864/1‑132 (MQ=255)
cTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAga < 2:123864/133‑2 (MQ=255)
gCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaa > 1:95656/1‑108 (MQ=255)
gCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaa < 2:95656/110‑3 (MQ=255)
|||
CAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGC > NC_000913_3_pae_tpiA/4367481‑4367630
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
