Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	169	80.7	2736954	99.0%	2709584	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,814	A→G	L134P (CTG→CCG)	yhaJ ←	DNA‑binding transcriptional activator YhaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,814	0	A	G	100.0%	162.4 / NA	45	L134P (CTG→CCG)	yhaJ	DNA‑binding transcriptional activator YhaJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/14); total (31/14)

CGCGGCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCAGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTC  >  NC_000913/3253684‑3253950

cgcgGCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCtg                                                                                                                                 >  1:644785/1‑140 (MQ=255)

    gCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGtgat                                                                                                                             >  1:787752/1‑140 (MQ=255)

        ggTAGACTTTCATAACCGTATAGAGCTGGCGCGATTTGATCCCCGACGAGAAAAAAAAAGCCATACCGGGCGCAAAAAAAATACCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGAtt                                                                                                                         <  2:838437/139‑1 (MQ=255)

                        cGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTgg                                                                                                         >  1:436826/1‑140 (MQ=255)

                          tataGGGTTGGCGCGAGTTGCTCCCCGACGAGAAACAAAAATGAATCCCGGGCGCAAAAAAAATTCCCCCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCt                                                                                                       <  2:436826/140‑1 (MQ=255)

                            taGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCttt                                                                                                     >  1:24605/1‑140 (MQ=255)

                             agagCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCACCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCtttt                                                                                                    >  2:1194494/1‑140 (MQ=255)

                               agCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGTGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgc                                                                                                  >  1:721573/1‑140 (MQ=255)

                               agCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgc                                                                                                  >  1:779540/1‑140 (MQ=255)

                                 cTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgccg                                                                                                >  1:523785/1‑140 (MQ=255)

                                   tGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgccgcc                                                                                              >  1:581871/1‑140 (MQ=255)

                                   tGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATCGGCTTTTgccgcc                                                                                              >  1:697150/1‑140 (MQ=255)

                                   tGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCAGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTgccgcc                                                                                              >  2:669399/1‑140 (MQ=255)

                                     cgcgAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAg                                                                                            >  1:1085543/1‑140 (MQ=255)

                                              aTCTCCGACGAGAAACAAAAATGCATACCCGGCGCGATAACAATACCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGAt                                                                                   <  1:156925/140‑1 (MQ=255)

                                              aTCCCCGACGAGAAACAAAAATGCATTCCCGGCGGAAAAACAATACCCGCCCCCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGAt                                                                                   <  1:1225963/140‑1 (MQ=255)

                                                        aGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGaaa                                                                         >  2:1118080/1‑140 (MQ=255)

                                                        aGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGaaa                                                                         >  1:683246/1‑140 (MQ=255)

                                                           aaCGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAg                                                                      >  2:54589/1‑140 (MQ=255)

                                                                    gCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTc                                                             >  2:2100/1‑140 (MQ=255)

                                                                     cATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCg                                                            >  1:789364/1‑140 (MQ=255)

                                                                     cATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCC‑GTTTGTCGATTAACGGGAAAAAGGCAGGTGTCgg                                                           >  1:165299/1‑140 (MQ=255)

                                                                       tccccGGCGCAAAAAAAATACCCCCCCGCCCCTGCCCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGt                                                          <  2:581871/137‑1 (MQ=255)

                                                                              cgcgATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAgcg                                                   >  2:352972/1‑140 (MQ=255)

                                                                               gcgATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCTCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAgcgc                                                  >  2:268012/1‑140 (MQ=255)

                                                                                cgATAAAAATACCCCCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCt                                                 <  1:78108/140‑1 (MQ=255)

                                                                                aaaaaaaaaTACCCCCCCCCCCCTGCCCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCt                                                 <  2:1085543/133‑1 (MQ=255)

                                                                                             cgcccgccCCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATgg                                    >  2:927189/1‑140 (MQ=255)

                                                                                               cccgcccCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTg                                  >  2:1286141/1‑140 (MQ=255)

                                                                                                ccgcccCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTga                                 >  1:149412/1‑140 (MQ=255)

                                                                                                 cgcccCTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGCAAAATGCAGGAGTCGGTACCAGCGCTTCGGTCCCAATGGTgaa                                >  2:340880/1‑139 (MQ=255)

                                                                                                    cccTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATg                             <  1:1118080/140‑1 (MQ=255)

                                                                                                    cccTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATg                             <  2:721573/140‑1 (MQ=255)

                                                                                                      cTGCTCCAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCg                           <  1:352972/140‑1 (MQ=255)

                                                                                                       tGCCCCACCCTTCCCCCCCCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGt                          <  1:1011273/140‑1 (MQ=255)

                                                                                                            cAGCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATCGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTccc                     <  2:697150/140‑1 (MQ=255)

                                                                                                              gCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCaa                   >  1:234558/1‑140 (MQ=255)

                                                                                                              gCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCaa                   >  2:296261/1‑140 (MQ=255)

                                                                                                              gCCGTTCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAAC‑GGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAAc                  >  1:1240329/1‑140 (MQ=255)

                                                                                                                  ttCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgc               >  1:1293980/1‑140 (MQ=255)

                                                                                                                   tCCCACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgcg              >  1:1144678/1‑140 (MQ=255)

                                                                                                                     ccACGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgcgcg            <  1:296261/140‑1 (MQ=255)

                                                                                                                       aCGCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACcgcgcgcg          >  1:356428/1‑140 (MQ=255)

                                                                                                                         gCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgag        <  2:779540/140‑1 (MQ=255)

                                                                                                                         gCCCCCGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCgag        >  2:1137405/1‑140 (MQ=255)

                                                                                                                              cGCCGGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCG‑CAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTc  >  2:376970/1‑140 (MQ=255)

CGCGGCAACGTAGACGTTCATTAACGTATAGAGCTTGCGCGAGTTGATCTCCGACGAGGAACGAAAATGCATATCCGGCGCGATAACAATATCCGCCCGCCCCTGCTCCAGCCGTTCCCACGCCCCCGCCAGCACTTCTGTGATGATTGCCAGTTGGGTATTGGCTTTTGCCGCCAGTTTGTCGATTAACGGGAAAAAGGCAGGTGTCGGTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTC  >  NC_000913/3253684‑3253950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A