Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,262	T→C	S130S (TCA→TCG)	cyuA ←	putative L‑cysteine desulfidase CyuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,262	0	T	C	100.0%	149.2 / NA	43	S130S (TCA→TCG)	cyuA	putative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/23); total (20/23)

ACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAC  >  NC_000913/3256126‑3256395

aCTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCgag                                                                                                                                    <  1:799044/140‑1 (MQ=255)

         cGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGAtt                                                                                                                           >  1:160206/1‑140 (MQ=255)

             ctctGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCAt                                                                                                                       <  1:82816/140‑1 (MQ=255)

                 gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  1:177053/140‑1 (MQ=255)

                 gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  2:753893/140‑1 (MQ=255)

                 gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  1:938694/140‑1 (MQ=255)

                 gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCa                                                                                                                   <  1:77023/140‑1 (MQ=255)

                         cGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCt                                                                                                           <  2:751862/140‑1 (MQ=255)

                         cGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCt                                                                                                           <  1:767547/140‑1 (MQ=255)

                                    tAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                                >  2:65978/1‑140 (MQ=255)

                                                  tCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCccgc                                                                                  >  1:434904/1‑140 (MQ=255)

                                                     tGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACATACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCcgccgc                                                                               >  2:980262/1‑140 (MQ=255)

                                                     tGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                                <  2:852873/123‑1 (MQ=255)

                                                     tGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACg                                                                                                >  1:852873/1‑123 (MQ=255)

                                                         tctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcagc                                                                           >  2:550411/1‑140 (MQ=255)

                                                         tctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcagc                                                                           <  1:65978/140‑1 (MQ=255)

                                                          ctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcagca                                                                          <  1:916810/140‑1 (MQ=255)

                                                                  gCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTg                                                                  <  2:434904/140‑1 (MQ=255)

                                                                   cacaATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTgg                                                                 >  2:176285/1‑140 (MQ=255)

                                                                         gTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcag                                                                            >  2:721346/1‑123 (MQ=255)

                                                                         gTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCcag                                                                            <  1:721346/123‑1 (MQ=255)

                                                                                    cgccgACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTgc                                                <  2:160206/140‑1 (MQ=255)

                                                                                                     cacGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               <  2:19329/140‑1 (MQ=255)

                                                                                                        gCCCACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCAc                            >  1:803524/1‑140 (MQ=255)

                                                                                                         cccACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACt                           >  1:379779/1‑140 (MQ=255)

                                                                                                         cccACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACt                           >  2:501815/1‑140 (MQ=255)

                                                                                                          ccACTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACtt                          <  2:379779/140‑1 (MQ=255)

                                                                                                             cTTCTCACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCa                       >  2:637266/1‑140 (MQ=255)

                                                                                                                ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGGCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               <  2:738633/129‑1 (MQ=255)

                                                                                                                ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               >  1:483563/1‑129 (MQ=255)

                                                                                                                ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               <  2:483563/129‑1 (MQ=255)

                                                                                                                ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAg                               >  1:738633/1‑129 (MQ=255)

                                                                                                                ctcACCGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGcc                    <  2:803524/140‑1 (MQ=255)

                                                                                                                    ccGTTCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGc                <  1:637266/140‑1 (MQ=255)

                                                                                                                        tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  2:74078/1‑140 (MQ=255)

                                                                                                                        tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            <  1:149952/140‑1 (MQ=255)

                                                                                                                        tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  2:426776/1‑140 (MQ=255)

                                                                                                                        tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  2:149952/1‑140 (MQ=255)

                                                                                                                        tCCAGACTTTGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTg            >  1:976713/1‑140 (MQ=255)

                                                                                                                                 tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTgg           >  2:745426/1‑132 (MQ=255)

                                                                                                                                 tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTgg           <  1:745426/132‑1 (MQ=255)

                                                                                                                                 tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAt        >  2:287717/1‑135 (MQ=255)

                                                                                                                                 tGGCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAt        <  1:287717/135‑1 (MQ=255)

                                                                                                                                  ggCGCGCGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAc  <  1:426776/140‑1 (MQ=255)

ACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAC  >  NC_000913/3256126‑3256395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A