Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,901,792	G→A	intergenic (‑10/‑256)	yoaL ← / → manX	protein YoaL/mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,901,792	0	G	A	100.0%	165.5 / NA	48	intergenic (‑10/‑256)	yoaL/manX	protein YoaL/mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/20); total (28/20)

GCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCT  >  NC_000913/1901666‑1901922

gCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGc                                                                                                                       <  1:809114/140‑1 (MQ=255)

gCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGc                                                                                                                       <  2:512237/140‑1 (MQ=255)

    agagTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAg                                                                                                                   <  1:725660/140‑1 (MQ=255)

                                   tGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACaa                                                                                    <  2:548190/140‑1 (MQ=255)

                                   tGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACaa                                                                                    <  2:45181/140‑1 (MQ=255)

                                    gATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAg                                                                                   <  1:546551/140‑1 (MQ=255)

                                                aCGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCt                                                                       >  1:305390/1‑140 (MQ=255)

                                                aCGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCt                                                                       >  2:264247/1‑140 (MQ=255)

                                                aCGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCATCCTTTGCAAACGAATGTGACAAGGATATTTTACCt                                                                       >  2:394557/1‑140 (MQ=255)

                                                  gTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCttt                                                                     >  1:264543/1‑140 (MQ=255)

                                                   tCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTc                                                                    >  2:640190/1‑140 (MQ=255)

                                                                      cGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGaa                                                 >  2:677080/1‑140 (MQ=255)

                                                                        ggCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAg                                               >  2:710326/1‑140 (MQ=255)

                                                                                cccTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTa                                       >  2:402948/1‑140 (MQ=255)

                                                                                cccTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTa                                       <  1:640190/140‑1 (MQ=255)

                                                                                cccTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTa                                       >  2:715309/1‑140 (MQ=255)

                                                                                     taCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACg                                     <  1:325288/137‑1 (MQ=255)

                                                                                     taCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACg                                     >  2:325288/1‑137 (MQ=255)

                                                                                     aaCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTCCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  <  2:390682/139‑1 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTGCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  <  2:576048/135‑1 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAAt                                         >  1:81762/1‑128 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAAt                                         <  2:81762/128‑1 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  >  1:576048/1‑135 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtca                             >  2:527713/1‑140 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtca                             >  2:243647/1‑140 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACA‑ATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACgg                                    >  2:311694/1‑132 (MQ=255)

                                                                                          cATGGTTGTTAAACGGGAGTTAAACA‑ATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACgg                                    <  1:311694/132‑1 (MQ=255)

                                                                                            tGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcatc                           >  1:408643/1‑140 (MQ=255)

                                                                                             ggTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTAc                                      >  1:35534/1‑128 (MQ=255)

                                                                                             ggTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTAc                                      <  2:35534/128‑1 (MQ=255)

                                                                                             ggTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcatca                          >  1:184030/1‑140 (MQ=255)

                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAGTGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  2:577007/1‑140 (MQ=255)

                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  <  2:324843/130‑1 (MQ=255)

                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  >  1:324843/1‑130 (MQ=255)

                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  1:224578/1‑140 (MQ=255)

                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  1:306356/1‑140 (MQ=255)

                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  1:662698/1‑140 (MQ=255)

                                                                                                 gttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcat                            <  1:511119/134‑1 (MQ=255)

                                                                                                 gttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcat                            >  2:511119/1‑134 (MQ=255)

                                                                                                 gttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATa                      >  2:325828/1‑140 (MQ=255)

                                                                                                       cGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAaataa                >  2:72346/1‑140 (MQ=255)

                                                                                                        gggAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAaataat               >  2:814632/1‑140 (MQ=255)

                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  2:562010/140‑1 (MQ=255)

                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  1:715309/140‑1 (MQ=255)

                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  1:325828/140‑1 (MQ=255)

                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  2:305390/140‑1 (MQ=255)

                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  2:306356/140‑1 (MQ=255)

                                                                                                                     atCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCt  >  1:158492/1‑140 (MQ=255)

GCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCT  >  NC_000913/1901666‑1901922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A