Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 116 49.4 1663006 99.2% 1649701 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,946,025 (A)7→6 intergenic (‑49/+90) ruvA ← / ← yobI Holliday junction branch migration complex subunit RuvA/uncharacterized protein YobI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,0190A.100.0% 241.9 / NA 57intergenic (‑43/+96)ruvA/yobIHolliday junction branch migration complex subunit RuvA/uncharacterized protein YobI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (28/29);  total (28/29)

ATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTG  >  NC_000913/1945882‑1946154
                                                                                                                                         |                                                                                                                                       
aTCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaa                                                                                                                                       <  2:4408/140‑1 (MQ=255)
  cGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaa                                                                                                                                     <  1:232342/140‑1 (MQ=255)
     cATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatg                                                                                                                                  <  2:796204/140‑3 (MQ=255)
     cATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatg                                                                                                                                  <  2:422508/140‑3 (MQ=255)
       tatGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgct                                                                                                                                >  2:32512/1‑136 (MQ=255)
        atGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTg                                                                                                                              <  1:319359/140‑1 (MQ=255)
          gCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGga                                                                                                                            <  2:248219/140‑1 (MQ=255)
          gCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGga                                                                                                                            >  2:325800/1‑140 (MQ=255)
               tCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatagat                                                                                                                       >  2:49911/1‑140 (MQ=255)
               tCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatagat                                                                                                                       >  1:335587/1‑140 (MQ=255)
                   aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatag                                                                                                                         <  2:604869/134‑1 (MQ=255)
                   aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatag                                                                                                                         >  1:604869/1‑134 (MQ=255)
                   aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATcc                                                                                                                   >  2:499111/1‑140 (MQ=255)
                   aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATcc                                                                                                                   >  2:429029/1‑140 (MQ=255)
                     ccTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAg                                                                                                                 >  2:475367/1‑140 (MQ=255)
                        aCGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGa                                                                                                              <  1:473992/140‑1 (MQ=255)
                        aCGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGa                                                                                                              >  2:697574/1‑140 (MQ=255)
                              cccACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATg                                                                                                        <  1:475367/140‑1 (MQ=255)
                               ccACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATga                                                                                                       >  2:505301/1‑140 (MQ=255)
                                      aaTTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAc                                                                                                <  1:505301/140‑1 (MQ=255)
                                             acCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAg                                                                                         >  2:86868/1‑140 (MQ=255)
                                                  cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                                                                    >  2:14097/1‑140 (MQ=255)
                                                  cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                                                                    >  1:664675/1‑140 (MQ=255)
                                                  cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                                                                    >  2:580562/1‑140 (MQ=255)
                                                    ggggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtctc                                                                                  >  1:711786/1‑140 (MQ=255)
                                                     gggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                                                 >  1:223142/1‑140 (MQ=255)
                                                        ttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATg                                                                              <  1:499111/140‑1 (MQ=255)
                                                         tgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGAT‑AAGAAAACTTGCGAGGTGTCTCGATGaa                                                                            <  1:32512/140‑1 (MQ=255)
                                                               tCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaa                                                                       <  2:223142/140‑1 (MQ=255)
                                                                     atgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 <  2:648961/140‑1 (MQ=255)
                                                                     atgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 <  2:217896/140‑1 (MQ=255)
                                                                      tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGc                                                                <  1:325800/140‑1 (MQ=255)
                                                                       gatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  2:711786/140‑1 (MQ=255)
                                                                       gatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  1:86868/140‑1 (MQ=255)
                                                                        atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcac                                                              >  1:343757/1‑140 (MQ=255)
                                                                        atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcac                                                              >  1:34395/1‑140 (MQ=255)
                                                                           ccTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGt                                                           <  1:697574/140‑1 (MQ=255)
                                                                              ctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtata                                                        <  2:801251/140‑1 (MQ=255)
                                                                                 aGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAAtt                                                     <  1:573032/140‑1 (MQ=255)
                                                                                   tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  2:651072/128‑1 (MQ=255)
                                                                                   tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               >  1:525768/1‑128 (MQ=255)
                                                                                   tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  2:525768/128‑1 (MQ=255)
                                                                                   tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               >  1:651072/1‑128 (MQ=255)
                                                                                     tGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTAtt                                                 >  2:85978/1‑140 (MQ=255)
                                                                                       ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 <  1:728966/122‑1 (MQ=255)
                                                                                       ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 >  2:728966/1‑122 (MQ=255)
                                                                                       ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                                               >  2:356682/1‑140 (MQ=255)
                                                                                       ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                                               <  1:536991/140‑1 (MQ=255)
                                                                                        cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTa                                              <  2:451544/140‑1 (MQ=255)
                                                                                         tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTAc                                             <  2:343757/140‑1 (MQ=255)
                                                                                           tCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                                           >  1:109384/1‑140 (MQ=255)
                                                                                                      tCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGa                                >  2:697704/1‑140 (MQ=255)
                                                                                                         tGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGc                             >  2:671910/1‑140 (MQ=255)
                                                                                                                tGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAtt                      >  2:390175/1‑140 (MQ=255)
                                                                                                                 gAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAttt                     <  1:671910/140‑1 (MQ=255)
                                                                                                                 gAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAttt                     <  2:48476/140‑1 (MQ=255)
                                                                                                                   aTCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTa                   <  1:356682/140‑1 (MQ=255)
                                                                                                                     cAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAat                 <  2:173029/140‑1 (MQ=255)
                                                                                                                       aaGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAatat               <  2:335587/140‑1 (MQ=255)
                                                                                                                               gCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATAtt       <  2:271881/140‑1 (MQ=255)
                                                                                                                                   tATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGt   >  1:324209/1‑140 (MQ=255)
                                                                                                                                    aTGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTg  >  2:214313/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTG  >  NC_000913/1945882‑1946154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A