Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,451	A→G	V61A (GTC→GCC)	nudB ←	dihydroneopterin triphosphate diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,451	0	A	G	100.0%	144.9 / NA	40	V61A (GTC→GCC)	nudB	dihydroneopterin triphosphate diphosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/16); total (24/16)

CGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGAT  >  NC_000913/1948315‑1948587

cgcAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCct                                                                                                                                       <  1:964749/140‑1 (MQ=255)

     gACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTcc                                                                                                                                  >  1:1132961/1‑140 (MQ=255)

          aaCCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTAc                                                                                                                             >  1:1078653/1‑140 (MQ=255)

               tGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgc                                                                                                                        >  2:79128/1‑140 (MQ=255)

               tGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgc                                                                                                                        >  2:368476/1‑140 (MQ=255)

                gATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgcg                                                                                                                       <  1:79128/140‑1 (MQ=255)

                gATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTcgcg                                                                                                                       <  2:1050141/140‑1 (MQ=255)

                    ccGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATg                                                                                                                   >  2:591420/1‑140 (MQ=255)

                            cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTg                                                                                                           >  1:225709/1‑140 (MQ=255)

                            cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTg                                                                                                           >  2:506168/1‑140 (MQ=255)

                            cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTg                                                                                                           >  2:161464/1‑140 (MQ=255)

                               gTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcgg                                                                                                        >  1:143832/1‑140 (MQ=255)

                                 cACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      <  1:298931/140‑1 (MQ=255)

                                 cACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      <  2:466131/140‑1 (MQ=255)

                                 cACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      >  1:540649/1‑140 (MQ=255)

                                      ccGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGtt                                                                                                 <  1:591420/140‑1 (MQ=255)

                                         gcgcATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      <  2:666745/132‑1 (MQ=255)

                                         gcgcATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTgcggcg                                                                                                      >  1:666745/1‑132 (MQ=255)

                                                 cGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTc                                                                                      <  1:495135/140‑1 (MQ=255)

                                                                 aaaaaaTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCACTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCa                                                                                    <  1:135217/126‑1 (MQ=255)

                                                                 aaaaaaTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCACTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCa                                                                                    >  2:135217/1‑126 (MQ=255)

                                                                   aaaaTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGa                                                                    <  1:161464/140‑1 (MQ=255)

                                                                                         gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGaa                                                           <  1:264648/127‑1 (MQ=255)

                                                                                         gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGaa                                                           >  2:264648/1‑127 (MQ=255)

                                                                                         gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCa                                              >  2:1102916/1‑140 (MQ=255)

                                                                                         gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCa                                              >  1:293895/1‑140 (MQ=255)

                                                                                         gcTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCa                                              >  1:331815/1‑140 (MQ=255)

                                                                                                   aaTTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCa                                    <  1:881772/140‑1 (MQ=255)

                                                                                                         ggTCAGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCa                              >  2:221259/1‑140 (MQ=255)

                                                                                                             aGTTGTTCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCAc                          >  2:1118716/1‑140 (MQ=255)

                                                                                                               ttgttCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCAccc                        >  2:333921/1‑140 (MQ=255)

                                                                                                                 gttCAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGt                      <  2:723737/140‑1 (MQ=255)

                                                                                                                    cAGCGACAACATCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTc                   >  2:224054/1‑140 (MQ=255)

                                                                                                                            acaTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtg           >  1:534956/1‑140 (MQ=255)

                                                                                                                            acaTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGCTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtg           >  1:189214/1‑140 (MQ=255)

                                                                                                                              aTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtgtg         <  1:676209/140‑1 (MQ=255)

                                                                                                                              aTCAATGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTtgtg         <  1:221259/140‑1 (MQ=255)

                                                                                                                                 aaTGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGt      >  2:484129/1‑140 (MQ=255)

                                                                                                                                  aTGGTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTa     <  2:126959/140‑1 (MQ=255)

                                                                                                                                     gTGGCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGAt  >  1:655044/1‑140 (MQ=255)

CGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGAT  >  NC_000913/1948315‑1948587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A