Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 252 48.5 1661298 98.8% 1641362 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,948,807 G→A N572N (AAC→AAT)  aspS ← aspartate‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,948,8070GA100.0% 107.0 / NA 32N572N (AAC→AAT) aspSaspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/14);  total (18/14)

AGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCG  >  NC_000913/1948674‑1948943
                                                                                                                                     |                                                                                                                                        
aGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCa                                                                                                                                    <  1:457441/140‑1 (MQ=255)
             cATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCtt                                                                                                                       <  1:200073/140‑1 (MQ=255)
                     ttCACTCAGTTGCCGCCTCTATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  1:454028/1‑140 (MQ=255)
                     ttCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  2:294716/1‑140 (MQ=255)
                     ttCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  1:437082/1‑140 (MQ=255)
                     ttCACTAAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGtcatc                                                                                                               >  2:173454/1‑140 (MQ=255)
                        aCTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGa                                                                                                            >  2:522494/1‑140 (MQ=255)
                                     cTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGccgccg                                                                                               <  2:437082/140‑1 (MQ=255)
                                               cgcgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCgg                                                                                     >  1:604838/1‑140 (MQ=255)
                                                 cgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGTTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGa                                                                                   >  2:678717/1‑140 (MQ=255)
                                                         ttCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATa                                                                           <  1:173454/140‑1 (MQ=255)
                                                                aTTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCAc                                                                    >  1:357581/1‑140 (MQ=255)
                                                                aTTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCAc                                                                    <  1:815324/140‑1 (MQ=255)
                                                                           atCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCg                                                         >  2:552501/1‑140 (MQ=255)
                                                                                 tATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCg                                                   >  1:645675/1‑140 (MQ=255)
                                                                                   ttCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGt                                                 >  1:282562/1‑140 (MQ=255)
                                                                                   ttCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGt                                                 <  1:294716/140‑1 (MQ=255)
                                                                                   ttCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGt                                                 <  2:272173/140‑1 (MQ=255)
                                                                                    tctcAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTc                                                >  2:332255/1‑140 (MQ=255)
                                                                                     ctcAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTca                                               <  1:355041/140‑1 (MQ=255)
                                                                                        aGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTcagca                                            <  2:357581/140‑1 (MQ=255)
                                                                                         gCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTcagcag                                           >  1:132408/1‑140 (MQ=255)
                                                                                                  acaACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAg                                  <  1:332255/140‑1 (MQ=255)
                                                                                                    aaCCTGAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGAc                                >  2:626498/1‑140 (MQ=255)
                                                                                                         gAATGCTCAGCTCAGCCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCa                           >  2:286334/1‑140 (MQ=255)
                                                                                                               tcagctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCg                     <  1:678717/140‑1 (MQ=255)
                                                                                                               tcagctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCg                     <  1:518034/140‑1 (MQ=255)
                                                                                                                cagctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGa                    >  2:151285/1‑140 (MQ=255)
                                                                                                                 agctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGaa                   >  1:198859/1‑140 (MQ=255)
                                                                                                                   ctcagcCAGTGCAGTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATg                 >  1:624148/1‑140 (MQ=255)
                                                                                                                                gTCGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtg    <  1:552501/140‑1 (MQ=255)
                                                                                                                                  cGGATTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCtgcgtgcg  <  2:454028/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
AGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCG  >  NC_000913/1948674‑1948943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A