Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
116 |
49.4 |
1663006 |
99.2% |
1649701 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,792,661 |
A→G |
E463E (GAA→GAG) |
gabD → |
NADP(+)‑dependent succinate‑semialdehyde dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,792,661 | 0 | A | G | 100.0%
| 39.6
/ NA
| 12 | E463E (GAA→GAG) | gabD | NADP(+)‑dependent succinate‑semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (5/7); total (5/7) |
GTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTC > NC_000913/2792534‑2792771
|
gTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTAt > 1:115662/1‑140 (MQ=255)
tGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATg < 1:524021/140‑1 (MQ=255)
aaGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCg < 2:147129/140‑1 (MQ=255)
ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTGTGGCATCGAAGATTACTTAGAAATCaa > 2:59339/1‑140 (MQ=255)
aaGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAatat < 2:763882/140‑1 (MQ=255)
tACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCgg < 2:161540/140‑1 (MQ=255)
cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTc > 1:28769/1‑140 (MQ=255)
aTCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGaa < 1:825674/140‑1 (MQ=255)
ttattTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAg > 2:282549/1‑140 (MQ=255)
ccAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAACATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATaa < 2:115662/140‑1 (MQ=255)
tGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAAt > 2:417799/1‑140 (MQ=255)
ggcATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTc < 1:282549/140‑1 (MQ=255)
|
GTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTC > NC_000913/2792534‑2792771
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
