Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,716	G→A	G328R (GGG→AGG)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,716	0	G	A	100.0%	183.4 / NA	52	G328R (GGG→AGG)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/24); total (28/24)

CGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAA  >  NC_000913/2793582‑2793847

cgGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAg                                                                                                                                <  2:272567/140‑1 (MQ=255)

 ggAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGa                                                                                                                               <  2:1009236/140‑1 (MQ=255)

       aaTGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGa                                                                                                                         <  1:303834/140‑1 (MQ=255)

             tGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACg                                                                                                                   >  1:604674/1‑140 (MQ=255)

             tGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACg                                                                                                                   <  1:980519/140‑1 (MQ=255)

             tGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAATTGAAAGACg                                                                                                                   >  1:779878/1‑140 (MQ=255)

              gCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACgg                                                                                                                  >  1:416891/1‑140 (MQ=255)

               ccGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGa                                                                                                                 >  2:935456/1‑140 (MQ=255)

               ccGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGa                                                                                                                 >  2:615040/1‑140 (MQ=255)

                cGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGAt                                                                                                                >  1:847318/1‑140 (MQ=255)

                cGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGAt                                                                                                                >  2:572062/1‑140 (MQ=255)

                          ggCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGAt                                                                                                      >  1:677101/1‑140 (MQ=255)

                                    gcggcACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGaaaaa                                                                                            <  1:572062/140‑1 (MQ=255)

                                     cggcACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAac                                                                                           >  2:834720/1‑140 (MQ=255)

                                        cACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACAcc                                                                                        >  1:500718/1‑140 (MQ=255)

                                          ccTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCg                                                                                      <  2:500718/140‑1 (MQ=255)

                                            tATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGga                                                                                    <  2:630208/140‑1 (MQ=255)

                                                cGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATc                                                                                <  1:415267/140‑1 (MQ=255)

                                                  ggTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAacac                                                                                         <  1:777786/129‑1 (MQ=255)

                                                  ggTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAacac                                                                                         >  2:777786/1‑129 (MQ=255)

                                                     aaCCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGa                                                                           <  2:604674/140‑1 (MQ=255)

                                                     aaCCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGa                                                                           >  1:1133247/1‑140 (MQ=255)

                                                       cccGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACg                                                                         >  2:514476/1‑140 (MQ=255)

                                                           aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:1133247/140‑1 (MQ=255)

                                                           aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:3284/140‑1 (MQ=255)

                                                           aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  1:834720/140‑1 (MQ=255)

                                                           aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  1:359222/140‑1 (MQ=255)

                                                           aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:847318/140‑1 (MQ=255)

                                                           aTTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcg                                                                     <  2:877969/140‑1 (MQ=255)

                                                            ttGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcgc                                                                    >  1:71337/1‑140 (MQ=255)

                                                               ccTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCggg                                                                 >  1:1083670/1‑140 (MQ=255)

                                                                 tGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCt                                                               <  1:935456/140‑1 (MQ=255)

                                                                  gCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTg                                                              >  1:1087259/1‑140 (MQ=255)

                                                                          gcgcTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgat                                                      <  2:416891/140‑1 (MQ=255)

                                                                             cTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgatgat                                                   >  2:949085/1‑140 (MQ=255)

                                                                             cTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgatgat                                                   <  2:864007/140‑1 (MQ=255)

                                                                                          aGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAg                                         <  1:471442/137‑1 (MQ=255)

                                                                                          aGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAg                                         >  2:471442/1‑137 (MQ=255)

                                                                                          aGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTg                                      >  1:179596/1‑140 (MQ=255)

                                                                                           ggTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGt                                     >  1:364926/1‑140 (MQ=255)

                                                                                               tttGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGa                                 <  2:1087259/140‑1 (MQ=255)

                                                                                                ttGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGaa                                >  1:459609/1‑140 (MQ=255)

                                                                                                     cAGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACgg                           <  1:188913/140‑1 (MQ=255)

                                                                                                      aGGAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGc                          >  2:262359/1‑140 (MQ=255)

                                                                                                        gAAAATCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGa                        >  2:1003285/1‑140 (MQ=255)

                                                                                                         aaaaTCTGCTGCAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGAt                       >  1:729417/1‑140 (MQ=255)

                                                                                                                 ctgcAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCAcaacaa               >  1:408313/1‑140 (MQ=255)

                                                                                                                  tgcAAAAAGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGAGATCACAACAAg              >  1:1105706/1‑140 (MQ=255)

                                                                                                                      aaaaGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg          <  1:949085/140‑1 (MQ=255)

                                                                                                                         aGCCAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACg       >  1:762135/1‑140 (MQ=255)

                                                                                                                           ccAACGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGcc     <  1:257617/140‑1 (MQ=255)

                                                                                                                              aCGATCTGAGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGTTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCaaa  >  2:237110/1‑140 (MQ=255)

CGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAA  >  NC_000913/2793582‑2793847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A