Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,798,154	A→G	R114R (CGT→CGC)	stpA ←	DNA‑binding transcriptional repressor StpA with RNA chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,798,154	0	A	G	100.0%	143.1 / NA	39	R114R (CGT→CGC)	stpA	DNA‑binding transcriptional repressor StpA with RNA chaperone activity
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/19); total (20/19)

AGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTT  >  NC_000913/2798019‑2798285

aGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGc                                                                                                                                 >  2:554917/1‑140 (MQ=255)

aGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGc                                                                                                                                 >  1:757983/1‑140 (MQ=255)

 gATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGcc                                                                                                                                <  2:113731/140‑1 (MQ=255)

   tGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCt                                                                                                                              >  2:270023/1‑140 (MQ=255)

      cGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGAc                                                                                                                           <  2:757983/140‑1 (MQ=255)

           aaCCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTc                                                                                                                      >  2:205687/1‑140 (MQ=255)

            aCCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTcc                                                                                                                     <  1:205687/140‑1 (MQ=255)

                     cTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAg                                                                                                            >  2:823868/1‑140 (MQ=255)

                       tGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGtt                                                                                                          <  1:577070/140‑1 (MQ=255)

                        gagaAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGttt                                                                                                         <  2:391991/140‑1 (MQ=255)

                          gaAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCa                                                                                                       >  2:561352/1‑140 (MQ=255)

                          gaAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCa                                                                                                       <  2:18802/140‑1 (MQ=255)

                               gACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGtt                                                                                                  <  2:367722/140‑1 (MQ=255)

                                aCGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTa                                                                                                 >  1:547246/1‑140 (MQ=255)

                                           gcCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGaa                                                                                      >  2:251319/1‑140 (MQ=255)

                                              cTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAAttt                                                                                   >  1:681551/1‑140 (MQ=255)

                                                     cGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTc                                                                            >  2:723136/1‑140 (MQ=255)

                                                         aTCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCgg                                                                                          <  1:822096/122‑1 (MQ=255)

                                                         aTCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCgg                                                                                          >  2:822096/1‑122 (MQ=255)

                                                           ccGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGa                                                                      <  2:115977/140‑1 (MQ=255)

                                                              gCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGAcgc                                                                   >  1:99834/1‑140 (MQ=255)

                                                                    gTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTgg                                                             <  1:270023/140‑1 (MQ=255)

                                                                      aaTTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCg                                                           <  1:723136/140‑1 (MQ=255)

                                                                             tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                    >  1:207077/1‑140 (MQ=255)

                                                                             tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                    >  1:764663/1‑140 (MQ=255)

                                                                               aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                  >  2:591685/1‑140 (MQ=255)

                                                                               aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                  >  2:641062/1‑140 (MQ=255)

                                                                                 gAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAg                                                >  1:445363/1‑140 (MQ=255)

                                                                                     tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                            <  2:35870/140‑1 (MQ=255)

                                                                                     tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                            <  1:641062/140‑1 (MQ=255)

                                                                                      cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                           >  1:404727/1‑140 (MQ=255)

                                                                                      cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                           <  2:445363/140‑1 (MQ=255)

                                                                                                 ttACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgcagcagc                                <  1:561352/140‑1 (MQ=255)

                                                                                                   aCCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                              <  1:823868/140‑1 (MQ=255)

                                                                                                   aCCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                              <  1:539495/140‑1 (MQ=255)

                                                                                                         tGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgc                                      >  1:583747/1‑126 (MQ=255)

                                                                                                         tGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgc                                      <  2:583747/126‑1 (MQ=255)

                                                                                                                   tGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATaa              <  1:251319/140‑1 (MQ=255)

                                                                                                                               ttCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGtt  >  2:360413/1‑140 (MQ=255)

AGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTT  >  NC_000913/2798019‑2798285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A