Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	143	98.3	3330986	99.3%	3307669	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,676,004	A→G	T407A (ACT→GCT)	yhjE →	putative transporter YhjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,676,004	0	A	G	98.6%	502.0 / ‑3.3	144	T407A (ACT→GCT)	yhjE	putative transporter YhjE
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (68/74); total (69/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 149 total aligned reads displayed.

TGACCTTCGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGACTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGC  >  NC_000913/3675867‑3676136

tGACCTTCGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGgct                                                                                                                                    >  1:1509264/1‑140 (MQ=255)

      tCGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGgctaacta                                                                                                                               <  2:1343538/139‑1 (MQ=255)

      tCGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTAc                                                                                                                              >  1:71543/1‑140 (MQ=255)

       cGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACg                                                                                                                             >  1:1000874/1‑140 (MQ=255)

       cGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACg                                                                                                                             >  1:974292/1‑140 (MQ=255)

           ccAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCt                                                                                                                         <  1:818857/140‑1 (MQ=255)

           ccAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCt                                                                                                                         <  1:1212763/140‑1 (MQ=255)

           ccAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCt                                                                                                                         <  2:735846/140‑1 (MQ=255)

              aTGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAgg                                                                                                                      >  2:1636360/1‑140 (MQ=255)

               tGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGgctaacta                                                                                                                               <  2:749067/130‑1 (MQ=255)

                   tGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCgg                                                                                                                 <  1:585546/140‑1 (MQ=255)

                   tGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCgg                                                                                                                 <  2:331352/140‑1 (MQ=255)

                   tGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCgg                                                                                                                 <  1:1118886/140‑1 (MQ=255)

                    gcgcTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGt                                                                                                                <  2:1509264/140‑1 (MQ=255)

                    gcgcTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGt                                                                                                                <  1:1636360/140‑1 (MQ=255)

                    gcgcTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGt                                                                                                                <  2:1000874/140‑1 (MQ=255)

                     cgcTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTg                                                                                                               <  2:1441798/140‑1 (MQ=255)

                       cTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGt                                                                                                                <  1:1370514/137‑1 (MQ=255)

                          ttACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCgg                                                                                                                 >  2:892750/1‑133 (MQ=255)

                          ttACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGtt                                                                                                          >  2:107669/1‑140 (MQ=255)

                                gagCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCt                                                                                                    >  2:902790/1‑140 (MQ=255)

                                  gCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCg                                                                                                                  >  2:840446/1‑124 (MQ=255)

                                  gCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTgg                                                                                                  >  2:1408315/1‑140 (MQ=255)

                                  gCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTgg                                                                                                  <  1:1002974/140‑1 (MQ=255)

                                      tttCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTggcggc                                                                                              >  2:471505/1‑140 (MQ=255)

                                            aCAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGAtggc                                                                                        <  1:376603/140‑1 (MQ=255)

                                                aaGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGAtggctggc                                                                                    <  1:107669/140‑1 (MQ=255)

                                                aaGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGAtggctggc                                                                                    >  2:484670/1‑140 (MQ=255)

                                                aaGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGACTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGAtgg                                                                                         >  1:44015/1‑135 (MQ=255)

                                                aaGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGACTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGAtgg                                                                                         <  2:44015/135‑1 (MQ=255)

                                                          cacCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTaa                                                                          >  2:35920/1‑140 (MQ=255)

                                                               gAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGcc                                                                     <  1:204710/140‑1 (MQ=255)

                                                               gAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGcc                                                                     >  2:1183764/1‑140 (MQ=255)

                                                               gAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGcc                                                                     >  2:1565749/1‑140 (MQ=255)

                                                                aGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGccc                                                                    <  1:484670/140‑1 (MQ=255)

                                                                  cATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTACAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCctg                                                                  >  2:601970/1‑140 (MQ=255)

                                                                    tCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTaa                                                                          >  1:342265/1‑130 (MQ=255)

                                                                    tCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTaa                                                                          <  2:342265/130‑1 (MQ=255)

                                                                    tCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCctgct                                                                >  2:270049/1‑140 (MQ=255)

                                                                         ctctTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGa                                                           >  2:86828/1‑140 (MQ=255)

                                                                          tctTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGAc                                                          >  1:600669/1‑140 (MQ=255)

                                                                               cAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATg                                                     >  1:1661180/1‑140 (MQ=255)

                                                                                 aCGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATgag                                                   >  2:39677/1‑140 (MQ=255)

                                                                                 aCGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATgag                                                   >  1:1326790/1‑140 (MQ=255)

                                                                                  cGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATgaga                                                  >  2:276997/1‑140 (MQ=255)

                                                                                  cGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATgaga                                                  >  2:959575/1‑140 (MQ=255)

                                                                                    tAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGAcg                                                >  2:900728/1‑140 (MQ=255)

                                                                                    tAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGAcg                                                >  2:1008690/1‑140 (MQ=255)

                                                                                    tAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGAcg                                                >  2:1043919/1‑140 (MQ=255)

                                                                                    tAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGAcg                                                >  2:1417108/1‑140 (MQ=255)

                                                                                    tAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATACCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGAcg                                                >  1:392778/1‑140 (MQ=255)

                                                                                      gCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGAcg                                                <  1:898924/138‑1 (MQ=255)

                                                                                       cGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCG‑CCTGCTGCTGACCCATGAGACGCGAc                                            >  1:863314/1‑140 (MQ=255)

                                                                                          cGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACAt                                          >  2:1358250/1‑140 (MQ=255)

                                                                                          cGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACAt                                          <  1:1565749/140‑1 (MQ=255)

                                                                                           gATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATc                                         <  1:532768/140‑1 (MQ=255)

                                                                                            aTTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCa                                        >  2:349155/1‑140 (MQ=255)

                                                                                            aTTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCa                                        >  2:1237701/1‑140 (MQ=255)

                                                                                             ttCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       <  1:291127/140‑1 (MQ=255)

                                                                                             ttCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       <  2:303645/140‑1 (MQ=255)

                                                                                             ttCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       <  2:622134/140‑1 (MQ=255)

                                                                                             ttCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       <  1:1418339/140‑1 (MQ=255)

                                                                                               ctcGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTc                                     <  1:349155/140‑1 (MQ=255)

                                                                                               ctcGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTACAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTc                                     <  1:601970/140‑1 (MQ=255)

                                                                                                 cGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGt                                   <  1:900728/140‑1 (MQ=255)

                                                                                                 cGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGt                                   <  1:1086913/140‑1 (MQ=255)

                                                                                                 cGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGt                                   >  2:1103881/1‑140 (MQ=255)

                                                                                                   gggCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTg                                 >  2:710199/1‑140 (MQ=255)

                                                                                                      cTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGtaa                              <  1:86828/140‑1 (MQ=255)

                                                                                                      cTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGtaa                              <  2:1661180/140‑1 (MQ=255)

                                                                                                       ttCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGtaat                             <  2:307820/140‑1 (MQ=255)

                                                                                                        tCCGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGtaata                            <  1:1408315/140‑1 (MQ=255)

                                                                                                          cGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAag                          <  1:59386/140‑1 (MQ=255)

                                                                                                          cGTTGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAag                          <  1:471505/140‑1 (MQ=255)

                                                                                                            ttGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAagag                        >  1:491744/1‑140 (MQ=255)

                                                                                                            ttGCGCCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAagag                        >  2:1605585/1‑140 (MQ=255)

                                                                                                               cgcCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       <  1:660454/122‑1 (MQ=255)

                                                                                                               cgcCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       >  1:918342/1‑122 (MQ=255)

                                                                                                               cgcCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAg                                       >  2:660454/1‑122 (MQ=255)

                                                                                                                gcCATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGaa                    <  1:1358250/140‑1 (MQ=255)

                                                                                                                  cATATATCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGaaaa                  <  1:276997/140‑1 (MQ=255)

                                                                                                                   atatatCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAg                 >  2:1419429/1‑140 (MQ=255)

                                                                                                                    tatatCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGtaataa                           <  2:930777/129‑1 (MQ=255)

                                                                                                                    tatatCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGtaataa                           >  1:930777/1‑129 (MQ=255)

                                                                                                                    tatatCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGc                >  1:112847/1‑140 (MQ=255)

                                                                                                                       atCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTg             <  1:63919/140‑1 (MQ=255)

                                                                                                                       atCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTg             <  2:1665372/140‑1 (MQ=255)

                                                                                                                       atCGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTg             >  2:1251519/1‑140 (MQ=255)

                                                                                                                         cGCAGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGAt           >  1:548247/1‑140 (MQ=255)

                                                                                                                            aGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATgcg        <  1:1008690/140‑1 (MQ=255)

                                                                                                                            aGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATgcg        <  2:594669/140‑1 (MQ=255)

                                                                                                                            aGCCTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATgcg        <  2:112847/140‑1 (MQ=255)

                                                                                                                              ccTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGa                     >  2:814480/1‑125 (MQ=255)

                                                                                                                              ccTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGa                     <  1:814480/125‑1 (MQ=255)

                                                                                                                              ccTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCt      >  2:1158830/1‑140 (MQ=255)

                                                                                                                               cTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTa     <  1:1237701/140‑1 (MQ=255)

                                                                                                                               cTGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTa     <  1:728700/140‑1 (MQ=255)

                                                                                                                                tGGTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATgcg        <  1:253595/136‑1 (MQ=255)

                                                                                                                                  gTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTg             >  2:574026/1‑129 (MQ=255)

                                                                                                                                  gTTGCAGGCTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGACGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGc  >  1:1311279/1‑140 (MQ=255)

TGACCTTCGGGCCAATGGGTGCGCTGTTACCAGAGCTGTTTCCGACAGAAGTGCGTTACACCGGAGCATCGTTCTCTTACAACGTAGCGTCGATTCTCGGGGCTTCCGTTGCGCCATATATCGCAGCCTGGTTGCAGACTAACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGC  >  NC_000913/3675867‑3676136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A