Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,734,952	T→C	V173A (GTT→GCT)	mepH →	peptidoglycan DD‑endopeptidase MepH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,734,952	0	T	C	100.0%	145.6 / NA	41	V173A (GTT→GCT)	mepH	peptidoglycan DD‑endopeptidase MepH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/19); total (22/19)

CTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGTTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAG  >  NC_000913/1734819‑1735085

ctTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCttatta                                                                                                                                 <  2:51573/140‑1 (MQ=255)

ctTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCttatta                                                                                                                                 <  2:670430/140‑1 (MQ=255)

 ttGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAG‑CATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACg                                                                                                                               >  2:78621/1‑140 (MQ=255)

          cgcACAAAGCGAAGGTACAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATaaa                                                                                                                       >  2:700354/1‑140 (MQ=255)

                  gCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGt                                                                                                               <  2:1148421/140‑1 (MQ=255)

                   cGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTg                                                                                                              >  1:1092054/1‑140 (MQ=255)

                          gCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTc                                                                                                       <  2:1092054/140‑1 (MQ=255)

                           cAAAAAGCTACGAAAGTGGCAATTAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACTCTTATAAAGATTTGGTGAAAATTCg                                                                                                      <  2:916249/140‑1 (MQ=255)

                                       aaaGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTAc                                                                                          <  1:708389/140‑1 (MQ=255)

                                         aGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACgg                                                                                        <  2:446994/140‑1 (MQ=255)

                                             gCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGaa                                                                                    >  2:787399/1‑140 (MQ=255)

                                                aTGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 <  1:84427/140‑1 (MQ=255)

                                                aTGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 <  2:1111461/140‑1 (MQ=255)

                                                         cTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACg                                                                                         >  1:355474/1‑123 (MQ=255)

                                                         cTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACg                                                                                         <  2:355474/123‑1 (MQ=255)

                                                         cTGATGCAGCAAATTGGTAAG‑CATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCAc                                                                       <  1:78621/140‑1 (MQ=255)

                                                            aTGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 >  1:553334/1‑128 (MQ=255)

                                                            aTGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 <  2:553334/128‑1 (MQ=255)

                                                             tGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTg                                                                    >  2:627591/1‑140 (MQ=255)

                                                                 gcaAATTGGTAAGCCGTATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTg                                                                >  2:644334/1‑140 (MQ=255)

                                                                          tAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAgcgc                                                       <  1:627591/140‑1 (MQ=255)

                                                                            aGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCa                                                     <  1:96425/140‑1 (MQ=255)

                                                                             gCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCaa                                                    <  1:1063067/140‑1 (MQ=255)

                                                                                atatCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCg                                                 >  1:762647/1‑140 (MQ=255)

                                                                                 tatCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGa                                                >  1:1140111/1‑140 (MQ=255)

                                                                                  atCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGaa                                               >  1:1194855/1‑140 (MQ=255)

                                                                                  atCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGAGGCCTGGCTTATTACGCTGATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGaa                                               >  2:1083981/1‑140 (MQ=255)

                                                                                   tCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAAc                                              >  1:489151/1‑140 (MQ=255)

                                                                                      ttGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTa                                           <  1:96941/140‑1 (MQ=255)

                                                                                      ttGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTa                                           >  1:935093/1‑140 (MQ=255)

                                                                                        ggggTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGt                                         >  1:248978/1‑140 (MQ=255)

                                                                                              gCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTg                                   >  2:437660/1‑140 (MQ=255)

                                                                                                     aCCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACg                            <  1:1100522/140‑1 (MQ=255)

                                                                                                      ccGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACgg                           <  2:248978/140‑1 (MQ=255)

                                                                                                               ggTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGt                  >  1:129166/1‑140 (MQ=255)

                                                                                                                gTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTc                 >  2:615774/1‑140 (MQ=255)

                                                                                                                 ttttGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCt                >  2:711082/1‑140 (MQ=255)

                                                                                                                  tttGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCtt               <  2:489151/140‑1 (MQ=255)

                                                                                                                      aTTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTc           >  1:985493/1‑140 (MQ=255)

                                                                                                                          cAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTa       >  2:528848/1‑140 (MQ=255)

                                                                                                                               gCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAg  >  1:1064629/1‑140 (MQ=255)

CTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGTTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAG  >  NC_000913/1734819‑1735085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A