Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,689	T→C	V160A (GTA→GCA)	ydjM →	putative inner membrane protein regulated by LexA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,689	0	T	C	100.0%	181.5 / NA	51	V160A (GTA→GCA)	ydjM	putative inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/27); total (24/27)

TGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGG  >  NC_000913/1810555‑1810824

tGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGa                                                                                                                                    <  2:363266/140‑1 (MQ=255)

    gATATGCTGACCCCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGcc                                                                                                                                <  2:632689/140‑1 (MQ=255)

    gATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGcc                                                                                                                                >  2:842868/1‑140 (MQ=255)

     atatGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGccc                                                                                                                               >  1:798868/1‑140 (MQ=255)

     atatGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGccc                                                                                                                               <  1:84705/140‑1 (MQ=255)

     atatGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGccc                                                                                                                               <  1:681954/140‑1 (MQ=255)

            gACCCCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCcattcat                                                                                                                        <  1:72018/140‑1 (MQ=255)

                   gccgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGACATGGATGCCCCATTCATTACCCga                                                                                                                 >  1:1099767/1‑140 (MQ=255)

                    ccgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCgag                                                                                                                >  2:310997/1‑140 (MQ=255)

                           tCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAgcg                                                                                                         >  1:842214/1‑140 (MQ=255)

                           tCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAgcg                                                                                                         >  2:1176029/1‑140 (MQ=255)

                             cccTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCt                                                                                                       <  1:598390/140‑1 (MQ=255)

                                 gCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTc                                                                                                   <  2:691249/140‑1 (MQ=255)

                                  ctctGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCg                                                                                                  >  2:694277/1‑140 (MQ=255)

                                          aTGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCAt                                                                                          <  1:842868/140‑1 (MQ=255)

                                                cTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCaaa                                                                                    <  1:310997/140‑1 (MQ=255)

                                                 tGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAAt                                                                                   >  2:161121/1‑140 (MQ=255)

                                                   gCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGa                                                                                 >  2:141166/1‑140 (MQ=255)

                                                      tttCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGACATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCa                                                                              <  2:1099767/140‑1 (MQ=255)

                                                         ccGCTTGCCTATCCTGGTT‑CCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATAc                                                                          <  1:506936/140‑1 (MQ=255)

                                                              tGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATTCCTTg                                                                      <  1:769164/140‑1 (MQ=255)

                                                                ccTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCa                                                                    <  1:1176029/140‑1 (MQ=255)

                                                                    tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATa                                                                           >  2:976818/1‑129 (MQ=255)

                                                                    tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATa                                                                           <  1:976818/129‑1 (MQ=255)

                                                                         gTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGtt                                                           >  2:1042389/1‑140 (MQ=255)

                                                                              ccAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATc                                                      >  2:663327/1‑140 (MQ=255)

                                                                                aaaaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCgg                                                    <  1:161121/140‑1 (MQ=255)

                                                                                 aaaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGc                                                   <  2:682469/140‑1 (MQ=255)

                                                                                  aaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCt                                                  <  1:1042389/140‑1 (MQ=255)

                                                                                       caaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCttatta                                             >  2:870495/1‑140 (MQ=255)

                                                                                       caaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCttatta                                             >  2:823902/1‑140 (MQ=255)

                                                                                        aaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTaa                                            >  1:417982/1‑140 (MQ=255)

                                                                                           caacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCa                                         <  1:823902/140‑1 (MQ=255)

                                                                                                ggAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAGCAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAgg                                    <  1:66598/140‑1 (MQ=255)

                                                                                                  aaCGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCCTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGtt                                  <  2:533278/140‑1 (MQ=255)

                                                                                                    cGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGa                                >  2:303946/1‑140 (MQ=255)

                                                                                                      ttttATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAAt                              <  1:303946/140‑1 (MQ=255)

                                                                                                                aTGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAgg                    >  2:874792/1‑140 (MQ=255)

                                                                                                                     attattTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa               >  2:229009/1‑140 (MQ=255)

                                                                                                                     attattTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa               >  2:775230/1‑140 (MQ=255)

                                                                                                                       tattTGTCTGGTCTGCATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGGTGAATACTAAAAAGGCAAAAAt             >  2:693188/1‑140 (MQ=255)

                                                                                                                         tttGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCa           <  2:876940/140‑1 (MQ=255)

                                                                                                                             tCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCtt       >  2:648287/1‑140 (MQ=255)

                                                                                                                              cTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt      <  2:599907/140‑1 (MQ=255)

                                                                                                                              cTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt      <  1:775230/140‑1 (MQ=255)

                                                                                                                              cTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt      <  1:229009/140‑1 (MQ=255)

                                                                                                                                 gTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTg   >  1:432974/1‑140 (MQ=255)

                                                                                                                                 gTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTg   <  2:798868/140‑1 (MQ=255)

                                                                                                                                 gTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTg   >  1:824743/1‑140 (MQ=255)

                                                                                                                                  tCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTgg  >  2:970972/1‑140 (MQ=255)

                                                                                                                                   cGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCAc          <  1:127180/131‑1 (MQ=255)

TGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGG  >  NC_000913/1810555‑1810824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A