Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,048,262	C→T	H1109Y (CAT→TAT)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,048,262	0	C	T	100.0%	125.5 / NA	36	H1109Y (CAT→TAT)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/15); total (21/15)

GCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCA  >  NC_000913/2048130‑2048396

gCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCAc                                                                                                                                 <  2:662172/140‑1 (MQ=255)

  aaCAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGc                                                                                                                               >  2:822932/1‑140 (MQ=255)

    cAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTg                                                                                                                             <  2:815787/140‑1 (MQ=255)

      gTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGaa                                                                                                                           <  1:85961/140‑1 (MQ=255)

      gTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGaa                                                                                                                           <  1:544339/140‑1 (MQ=255)

            gATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTaa                                                                                                                     >  1:766596/1‑140 (MQ=255)

                 gTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAg                                                                                                                >  2:861017/1‑140 (MQ=255)

                     aaTCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCggg                                                                                                            <  1:689943/140‑1 (MQ=255)

                          tcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGc                                                                                                       >  1:904544/1‑140 (MQ=255)

                                    gggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGt                                                                                                >  1:301818/1‑137 (MQ=255)

                                    gggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGt                                                                                                <  2:301818/137‑1 (MQ=255)

                                                aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTggg                                                                                 >  1:87049/1‑140 (MQ=255)

                                                     cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCaa                                                                                        <  1:330507/128‑1 (MQ=255)

                                                     cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCaa                                                                                        >  2:330507/1‑128 (MQ=255)

                                                     cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaata                                                                            >  1:636248/1‑140 (MQ=255)

                                                     cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaata                                                                            >  2:632190/1‑140 (MQ=255)

                                                     cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGt                                                                                >  2:701004/1‑136 (MQ=255)

                                                     cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGt                                                                                <  1:701004/136‑1 (MQ=255)

                                                               caGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAg                                                                  >  2:328072/1‑140 (MQ=255)

                                                                                   tACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCa                                                         >  2:1036384/1‑129 (MQ=255)

                                                                                   tACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCa                                                         <  1:1036384/129‑1 (MQ=255)

                                                                                   tACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCa                                                         >  2:421316/1‑129 (MQ=255)

                                                                                   tACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCa                                                         <  1:421316/129‑1 (MQ=255)

                                                                                         tGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTgg                                        <  1:75499/140‑1 (MQ=255)

                                                                                               tAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACa                                  <  1:501663/140‑1 (MQ=255)

                                                                                               tAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGA‑‑‑‑‑AAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGcc                             >  1:1197505/1‑140 (MQ=255)

                                                                                                         gCCATCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGc                        <  1:606392/140‑1 (MQ=255)

                                                                                                             tCACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAg                    >  1:1186210/1‑140 (MQ=255)

                                                                                                              cACTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAgg                   <  1:328072/140‑1 (MQ=255)

                                                                                                               aCTCAGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGt                  >  2:272989/1‑140 (MQ=255)

                                                                                                                   aGGCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTc              >  2:465416/1‑140 (MQ=255)

                                                                                                                    ggCCAATGGGGAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTcc             >  1:683342/1‑140 (MQ=255)

                                                                                                                           ggggAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGat      >  1:851467/1‑140 (MQ=255)

                                                                                                                           ggggAAGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGat      <  1:272989/140‑1 (MQ=255)

                                                                                                                               aaGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGGCTCGGCTCAGGTTTTTCTGCAGATATCa  >  2:1128898/1‑140 (MQ=255)

                                                                                                                               aaGCGTATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCa  >  2:6147/1‑140 (MQ=255)

GCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCA  >  NC_000913/2048130‑2048396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A