Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,141,905	A→G	V104A (GTG→GCG)	dcd ←	dCTP deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,141,905	0	A	G	100.0%	122.7 / NA	35	V104A (GTG→GCG)	dcd	dCTP deaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/15); total (20/15)

GCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTC  >  NC_000913/2141771‑2142038

gcgcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGa                                                                                                                                  >  2:834703/1‑140 (MQ=255)

 cgcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGATGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGAt                                                                                                                                 >  1:236191/1‑140 (MQ=255)

                  cGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCAcc                                                                                                                <  2:954823/140‑1 (MQ=255)

                    gAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCATATCGGCTGGCAGCGTCACCGa                                                                                                              >  1:530406/1‑140 (MQ=255)

                            gAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCg                                                                                                      >  2:212325/1‑140 (MQ=255)

                                    gCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcc                                                                                              <  2:341817/140‑1 (MQ=255)

                                    gCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcc                                                                                              >  2:94767/1‑140 (MQ=255)

                                    gCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcc                                                                                              >  1:407288/1‑140 (MQ=255)

                                          tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                                                        >  1:42039/1‑140 (MQ=255)

                                           gCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcca                                                                                       <  2:485707/140‑1 (MQ=255)

                                            cAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgccag                                                                                      <  2:42039/140‑1 (MQ=255)

                                              accagaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGct                                                                                    <  1:94767/140‑1 (MQ=255)

                                               ccagaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGctc                                                                                   >  1:1005494/1‑140 (MQ=255)

                                                  gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                                                                >  1:313552/1‑140 (MQ=255)

                                                  gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                                                                >  2:738559/1‑140 (MQ=255)

                                                  gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                                                                >  1:1107093/1‑140 (MQ=255)

                                                    ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                                                              >  2:970301/1‑140 (MQ=255)

                                                    ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                                                              >  1:1012082/1‑140 (MQ=255)

                                                    ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                                                              >  1:606144/1‑140 (MQ=255)

                                                                 aTGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGaa                                                                 <  1:212325/140‑1 (MQ=255)

                                                                          gcgGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGcc                                                        <  1:145270/140‑1 (MQ=255)

                                                                            ggTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCt                                                      >  1:557154/1‑140 (MQ=255)

                                                                                     cACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGaa                                             <  2:557154/140‑1 (MQ=255)

                                                                                      aCCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAAc                                            <  1:834703/140‑1 (MQ=255)

                                                                                        cATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGTCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGa                                          >  1:358319/1‑140 (MQ=255)

                                                                                         aTCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGAt                                         <  2:1107093/140‑1 (MQ=255)

                                                                                           cAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGAtct                                       >  1:1042934/1‑140 (MQ=255)

                                                                                                        gcCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCAc                          >  1:287255/1‑140 (MQ=255)

                                                                                                           aGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCAcgcg                       >  2:821972/1‑140 (MQ=255)

                                                                                                            gTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCgg                      >  1:79193/1‑140 (MQ=255)

                                                                                                                          tCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCt        <  1:738559/140‑1 (MQ=255)

                                                                                                                          tCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCt        <  1:970301/140‑1 (MQ=255)

                                                                                                                          tCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCt        <  1:1063931/140‑1 (MQ=255)

                                                                                                                              gccagccCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACt    <  2:1042934/140‑1 (MQ=255)

                                                                                                                                cagccCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTc  <  2:287255/140‑1 (MQ=255)

GCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTC  >  NC_000913/2141771‑2142038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A