Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,143,407	T→C	F48L (TTC→CTC)	dgcE →	putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,143,407	0	T	C	100.0%	130.8 / NA	38	F48L (TTC→CTC)	dgcE	putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/12); total (26/12)

CCCTGCCCCACCCGCTGCTTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGTTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCGCTGAACATGACCT  >  NC_000913/2143297‑2143542

ccctgcccCACCCGCTGCTTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGttt                                                                                                            >  1:821582/1‑140 (MQ=255)

ccctgcccCACCCGCTGCTTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGttt                                                                                                            >  1:771208/1‑140 (MQ=255)

          cccGCTGCTTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATg                                                                                                  >  2:145819/1‑140 (MQ=255)

          cccGCTGCTTCACCTGGACAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATg                                                                                                  >  2:256886/1‑140 (MQ=255)

               tgctTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCggg                                                                                             >  1:271377/1‑140 (MQ=255)

               tgctTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCggg                                                                                             >  2:581363/1‑140 (MQ=255)

                    cACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGCGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAt                                                                                        >  1:156955/1‑140 (MQ=255)

                           tCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCg                                                                                 >  2:85031/1‑140 (MQ=255)

                               tttaggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGaa                                                                             <  2:271377/140‑1 (MQ=255)

                               tttaggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGaa                                                                             <  1:120811/140‑1 (MQ=255)

                               tttaggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGaa                                                                             >  2:650109/1‑140 (MQ=255)

                                    gtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgcg                                                                        >  2:439625/1‑140 (MQ=255)

                                    gtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgcg                                                                        <  1:650109/140‑1 (MQ=255)

                                        agTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGa                                                                    >  2:242832/1‑140 (MQ=255)

                                              gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGtt                                                              <  2:77247/140‑1 (MQ=255)

                                               tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTc                                                             >  1:778064/1‑140 (MQ=255)

                                               tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTc                                                             >  1:417241/1‑140 (MQ=255)

                                                        aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGaaa                                                    >  1:281122/1‑140 (MQ=255)

                                                        aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGaaa                                                    <  1:242832/140‑1 (MQ=255)

                                                             tttCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATcg                                               >  2:185585/1‑140 (MQ=255)

                                                              ttCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATcgc                                              >  1:654237/1‑140 (MQ=255)

                                                               tctcGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATcgcc                                             >  2:694936/1‑140 (MQ=255)

                                                                 tcGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATcgccgc                                           >  2:746135/1‑140 (MQ=255)

                                                                        aGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATcgc                                              <  1:695746/130‑1 (MQ=255)

                                                                        aGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATcgc                                              >  2:695746/1‑130 (MQ=255)

                                                                           tttCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTg                                 <  1:95180/140‑1 (MQ=255)

                                                                            ttCGCAGTTTGACACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGc                                <  2:139535/140‑1 (MQ=255)

                                                                             tCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCt                               >  1:454556/1‑140 (MQ=255)

                                                                             tCGCAGTTTGGCACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGACGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCt                               >  1:212515/1‑140 (MQ=255)

                                                                                        cACCCAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTccacca                    <  2:654237/140‑1 (MQ=255)

                                                                                           ccAACTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCt                 >  1:699402/1‑140 (MQ=255)

                                                                                             aaCTCGCCCCACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCg               <  1:426529/140‑1 (MQ=255)

                                                                                                   ccccACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCGCTGAAc         <  2:821582/140‑1 (MQ=255)

                                                                                                    cccACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTc                         <  2:127728/123‑1 (MQ=255)

                                                                                                    cccACTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTc                         >  1:127728/1‑123 (MQ=255)

                                                                                                       aCTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCGCTGAACATGa     >  2:271919/1‑140 (MQ=255)

                                                                                                       aCTGTGGCTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCGCTGAACATGa     >  2:681935/1‑140 (MQ=255)

                                                                                                          gtgGCTCCCGACGTCCATTATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCGCTGAACATGACCt  >  2:156723/1‑140 (MQ=255)

CCCTGCCCCACCCGCTGCTTCACCTGGTCAGTTTAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGGCACCCAACTCGCCCCACTGTGGTTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTGGGAAATATCGCCGCATCCATCCTGCTTTTTTCCACCAGCTCGCTGAACATGACCT  >  NC_000913/2143297‑2143542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A