Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,523	A→G	T593A (ACC→GCC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,523	0	A	G	98.0%	173.2 / ‑4.6	51	T593A (ACC→GCC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (28/22); total (28/23)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGA  >  NC_000913/2416386‑2416655

aaCAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGcc                                                                                                                                    <  1:1173193/140‑1 (MQ=255)

 aCAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTccgccg                                                                                                                                   <  2:383854/140‑1 (MQ=255)

  cAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCgg                                                                                                                                  <  1:767653/140‑1 (MQ=255)

   aGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGt                                                                                                                                 >  1:379345/1‑140 (MQ=255)

        tacgtttacgGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCgg                                                                                                                                  <  2:984469/134‑1 (MQ=255)

        tacgtttacgGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCgg                                                                                                                                  >  1:984469/1‑134 (MQ=255)

              tacgGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGc                                                                                                                      <  1:1165434/140‑1 (MQ=255)

               acgGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCa                                                                                                                     >  1:1027422/1‑140 (MQ=255)

               acgGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCa                                                                                                                     <  2:379345/140‑1 (MQ=255)

                         tgCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGa                                                                                                             >  1:1006259/1‑138 (MQ=255)

                         tgCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGa                                                                                                             <  2:1006259/138‑1 (MQ=255)

                         tgCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACg                                                                                                           >  1:935660/1‑140 (MQ=255)

                             aTCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGa                                                                                                       >  2:1215923/1‑140 (MQ=255)

                                 aCCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGaaaaa                                                                                                   >  2:1115688/1‑140 (MQ=255)

                                      gATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTcg                                                                                              >  2:828355/1‑140 (MQ=255)

                                          cGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGaa                                                                                          <  1:1215923/140‑1 (MQ=255)

                                            aCCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGc                                                                                        >  2:902531/1‑140 (MQ=255)

                                              cGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCaa                                                                                      <  1:183302/140‑1 (MQ=255)

                                              cGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCaa                                                                                      <  2:1027422/140‑1 (MQ=255)

                                                cTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCAGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACt                                                                                    >  1:61549/1‑140 (MQ=255)

                                                 tGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTc                                                                                   >  1:593589/1‑140 (MQ=255)

                                                   aaCAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGt                                                                                 <  1:168383/140‑1 (MQ=255)

                                                   aaCAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGt                                                                                 <  1:251627/140‑1 (MQ=255)

                                                        cTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGgc                                                                            >  1:787002/1‑140 (MQ=255)

                                                         tGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGgcg                                                                           <  2:349799/140‑1 (MQ=255)

                                                         tGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGgcg                                                                           <  1:902531/140‑1 (MQ=255)

                                                           gCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCa                                                                         >  1:216185/1‑140 (MQ=255)

                                                             aGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAgg                                                                       >  2:138776/1‑140 (MQ=255)

                                                                 aTCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGaaaa                                                                   <  2:216185/140‑1 (MQ=255)

                                                                 aTCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCAGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGaaaa                                                                   <  2:61549/140‑1 (MQ=255)

                                                                  tCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGaaaaa                                                                  >  2:246850/1‑140 (MQ=255)

                                                                  tCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGCTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGaaaaa                                                                  >  2:1203041/1‑140 (MQ=255)

                                                                      gATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTc                                                              <  1:828355/140‑1 (MQ=255)

                                                                           aGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTcctgac                                                         <  1:246850/140‑1 (MQ=255)

                                                                             tCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTcctgacct                                                       >  1:447881/1‑140 (MQ=255)

                                                                                  tGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCtgatga                                                  >  2:603833/1‑140 (MQ=255)

                                                                                  tGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCtgacga                                                  >  1:528583/1‑140 (MQ=255)

                                                                                          cTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGt                                          >  2:1203965/1‑140 (MQ=255)

                                                                                           tGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTc                                         <  1:603833/140‑1 (MQ=255)

                                                                                                     ggTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTa                               <  2:787002/140‑1 (MQ=255)

                                                                                                       tATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTc                                         >  1:542777/1‑128 (MQ=255)

                                                                                                       tATCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTc                                         <  2:542777/128‑1 (MQ=255)

                                                                                                        aTCGAACCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTacga                            <  1:823925/140‑1 (MQ=255)

                                                                                                            aaCCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGc                                      >  1:1190277/1‑126 (MQ=255)

                                                                                                            aaCCGCGCGTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGc                                      <  2:1190277/126‑1 (MQ=255)

                                                                                                                 cgcgTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGt                   >  1:208924/1‑140 (MQ=255)

                                                                                                                   cgTTGCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTaa                 >  2:1014615/1‑140 (MQ=255)

                                                                                                                       gCTATGCTCTCCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGACGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGc             <  2:528583/140‑1 (MQ=255)

                                                                                                                                tcCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGc    <  1:1203965/140‑1 (MQ=255)

                                                                                                                                tcCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGc    >  2:30108/1‑140 (MQ=255)

                                                                                                                                tcCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGc    >  1:618902/1‑140 (MQ=255)

                                                                                                                                tcCTACTCCGCCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTCACGTTGc    >  1:77723/1‑140 (MQ=255)

                                                                                                                                  cTACTCCGCCGGTACTTCTGGCGCAGGCAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGa  >  1:368514/1‑140 (MQ=255)

AACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGA  >  NC_000913/2416386‑2416655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A