Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,680	(G)_7→8	coding (212/756 nt)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,673	1	.	G	98.8%	281.4 / ‑3.5	80	coding (219/756 nt)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (32/47); total (32/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCG  >  NC_000913/2999536‑2999796

ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gg                                                                                                                           >  1:537609/1‑140 (MQ=255)

 gcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                                                          <  2:1213019/140‑1 (MQ=255)

 gcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                                                          >  1:590671/1‑140 (MQ=255)

 gcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                                                          >  1:74180/1‑140 (MQ=255)

     ggATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggggggg                                                                                                                      >  1:630655/1‑140 (MQ=255)

     ggATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggggggg                                                                                                                      <  1:29106/140‑1 (MQ=255)

           tttCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATg                                                                                                                 <  2:733133/140‑1 (MQ=255)

           tttCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATg                                                                                                                 >  1:765752/1‑140 (MQ=255)

               ggTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGt                                                                                                             <  2:223441/140‑1 (MQ=255)

               ggTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGt                                                                                                             <  1:685371/140‑1 (MQ=255)

               ggTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGt                                                                                                             <  1:180032/140‑1 (MQ=255)

                 tACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTc                                                                                                           <  1:301152/140‑1 (MQ=255)

                    cGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGt                                                                                                        >  2:58169/1‑140 (MQ=255)

                    cGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGt                                                                                                        >  1:896681/1‑140 (MQ=255)

                    cGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCC‑TTCAGt                                                                                                        >  1:4819/1‑139 (MQ=255)

                    cGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCC‑TTCAGt                                                                                                        <  2:4819/139‑1 (MQ=255)

                      cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGGTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTcg                                                                                                      >  2:149742/1‑140 (MQ=255)

                        gatgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCgcgc                                                                                                   <  1:279042/140‑1 (MQ=255)

                          tgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGcc                                                                                                  <  2:426190/140‑1 (MQ=255)

                            aCGGTGTAACCGATCCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                <  2:747973/140‑1 (MQ=255)

                                  tAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCttt                                                                                          <  2:90939/140‑1 (MQ=255)

                                   aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGA‑TTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                                                        >  2:1054101/1‑140 (MQ=255)

                                    aCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGCAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa                                                                                        <  2:1128747/140‑1 (MQ=255)

                                     ccGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTAc                                                                                       <  2:1163356/140‑1 (MQ=255)

                                      cGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg                                                                                      <  2:271540/140‑1 (MQ=255)

                                        aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    <  1:911569/140‑1 (MQ=255)

                                        aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    <  2:1081543/140‑1 (MQ=255)

                                        aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    >  2:176956/1‑140 (MQ=255)

                                        aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    >  1:1070340/1‑140 (MQ=255)

                                        aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    >  1:264289/1‑140 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                >  1:1127873/1‑126 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCttt                                                                                          >  1:66627/1‑132 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCttt                                                                                          <  2:66627/132‑1 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                                                  <  2:537609/140‑1 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                                                  <  1:726976/140‑1 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                                                  >  2:1110909/1‑140 (MQ=255)

                                          gCGGTTTTGGTCGTTGATTTGGCGGTTGACTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                <  2:1127873/126‑1 (MQ=255)

                                                ttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCg                                                                            <  1:1110909/140‑1 (MQ=255)

                                                   gTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtgg                                                                         >  2:258373/1‑140 (MQ=255)

                                                       ttGATTTGTCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                                                     <  2:477968/140‑1 (MQ=255)

                                                        tGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    <  1:394968/124‑1 (MQ=255)

                                                        tGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                    >  2:394968/1‑124 (MQ=255)

                                                            ttGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa                                                                <  1:1148315/140‑1 (MQ=255)

                                                             tGGCGGTTGA‑TTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                              <  1:1054101/140‑1 (MQ=255)

                                                              ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                              <  1:176956/140‑1 (MQ=255)

                                                              ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                              <  2:368605/140‑1 (MQ=255)

                                                                  gTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGat                                                          >  2:1108815/1‑140 (MQ=255)

                                                                   ttGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGata                                                         <  1:1108815/140‑1 (MQ=255)

                                                                    tGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGatat                                                        >  2:2950/1‑140 (MQ=255)

                                                                    tGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGatat                                                        >  1:377406/1‑140 (MQ=255)

                                                                     gATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGAtata                                                       <  2:925362/140‑1 (MQ=255)

                                                                             tgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATc                                                >  2:258737/1‑140 (MQ=255)

                                                                              gtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccc                                              <  1:149742/140‑1 (MQ=255)

                                                                               tAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcccc                                             >  2:680314/1‑140 (MQ=255)

                                                                                aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccccc                                            >  2:153197/1‑140 (MQ=255)

                                                                                aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCCGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                              >  2:1102785/1‑122 (MQ=255)

                                                                                aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCCGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                              <  1:1102785/122‑1 (MQ=255)

                                                                                     tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGttt                                       >  1:377034/1‑140 (MQ=255)

                                                                                      gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                      >  2:926654/1‑140 (MQ=255)

                                                                                      gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                      <  1:58169/140‑1 (MQ=255)

                                                                                       cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                     >  2:1029101/1‑140 (MQ=255)

                                                                                       cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                     >  1:133526/1‑140 (MQ=255)

                                                                                       cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                     >  2:512754/1‑140 (MQ=255)

                                                                                        tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                    >  2:85370/1‑140 (MQ=255)

                                                                                        tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                    <  1:258373/140‑1 (MQ=255)

                                                                                        tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                    >  1:370639/1‑140 (MQ=255)

                                                                                        tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                    <  1:512754/140‑1 (MQ=255)

                                                                                               cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaa                             >  1:464585/1‑140 (MQ=255)

                                                                                               cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaa                             >  1:554825/1‑140 (MQ=255)

                                                                                                gCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaaa                            <  1:1120862/140‑1 (MQ=255)

                                                                                                gCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaaa                            <  1:2950/140‑1 (MQ=255)

                                                                                                 ccccACCCAGTTTTAGTTTCTGACCAACATAAATGGTGCAAG‑GGGGGGGAAATGCCGTTCAGTTGCGCCTGTTCTTTTACGCTGGTTCCCGGGGGGCGCGAACGCCGAACTAGCGGATCCCCCCGTTTCCCGGAGTAAAc                           >  2:1120862/1‑140 (MQ=255)

                                                                                                     acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAg                       >  1:630525/1‑140 (MQ=255)

                                                                                                      cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc                      <  2:305175/140‑1 (MQ=255)

                                                                                                        caGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCg                    <  2:441090/140‑1 (MQ=255)

                                                                                                        caGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCAAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCg                    <  1:1217051/140‑1 (MQ=255)

                                                                                                             ttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa               <  2:377406/140‑1 (MQ=255)

                                                                                                             ttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa               <  2:370639/140‑1 (MQ=255)

                                                                                                             ttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa               <  2:74180/140‑1 (MQ=255)

                                                                                                             ttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa               <  2:1119321/140‑1 (MQ=255)

                                                                                                              tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa               <  2:630655/140‑1 (MQ=255)

                                                                                                              tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa               <  1:258737/140‑1 (MQ=255)

                                                                                                                     tGACCAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTg       >  1:186928/1‑140 (MQ=255)

                                                                                                                        ccAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTAt    <  2:377034/140‑1 (MQ=255)

                                                                                                                        ccAACTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTAt    <  1:680314/140‑1 (MQ=255)

                                                                                                                          aaCTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCg  <  2:133526/140‑1 (MQ=255)

                                                                                                                          aaCTTCAATGGTGTAAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCg  <  1:1029101/140‑1 (MQ=255)

GGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCG  >  NC_000913/2999536‑2999796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A