Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	126	206.4	6980590	99.2%	6924745	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,680	(G)_7→8	coding (212/756 nt)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,673	1	.	G	96.6%	623.3 / 10.7	177	coding (219/756 nt)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand): ref base . (2/4); new base G (78/93); total (80/97)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 196 total aligned reads displayed.

GCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCT  >  NC_000913/2999534‑2999807

gcggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTaa                                                                                                                                           >  1:1614250/1‑140 (MQ=255)

gcggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTaa                                                                                                                                           >  2:2391435/1‑140 (MQ=255)

  ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gg                                                                                                                                       <  1:2278708/140‑1 (MQ=255)

  ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gg                                                                                                                                       >  1:3165013/1‑140 (MQ=255)

  ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑gg                                                                                                                                       <  1:1785791/140‑1 (MQ=255)

   gcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                                                                      <  2:1890671/140‑1 (MQ=255)

   gcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                                                                      <  1:2365166/140‑1 (MQ=255)

   gcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg                                                                                                                                      <  1:1893069/140‑1 (MQ=255)

     cAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGGTTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg‑gg                                                                                                                                   >  2:1778326/1‑140 (MQ=255)

       ggATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg‑gggg                                                                                                                                 >  2:365508/1‑140 (MQ=255)

       ggATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑ggg‑gggg                                                                                                                                 >  2:933307/1‑140 (MQ=255)

              ttCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGc                                                                                                                           >  1:472734/1‑140 (MQ=255)

              ttCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGc                                                                                                                           >  2:1711174/1‑140 (MQ=255)

                  gTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGtt                                                                                                                       >  2:2481573/1‑140 (MQ=255)

                  gTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGtt                                                                                                                       >  2:2665975/1‑140 (MQ=255)

                     ccGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAg                                                                                                                    >  1:3384489/1‑140 (MQ=255)

                      cGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGATGCTTTACGGGTAATACTGCTACTTTTCCCCCCACCCAGTTTTAGGTTGTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGGAACGCCGTTctgg                                                                                                                   >  2:1468488/1‑137 (MQ=255)

                        cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTcg                                                                                                                 >  2:1532360/1‑140 (MQ=255)

                        cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTcg                                                                                                                 <  2:849312/140‑1 (MQ=255)

                             gaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCgcgc                                                                                                              <  2:3244257/138‑1 (MQ=255)

                              aCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                           <  2:2975450/140‑1 (MQ=255)

                              aCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                           <  1:2391435/140‑1 (MQ=255)

                              aCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAg                                                                                                           <  1:1017152/140‑1 (MQ=255)

                                  tgtAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCt                                                                                                       >  2:3284707/1‑140 (MQ=255)

                                   gtAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCtt                                                                                                      <  1:739342/140‑1 (MQ=255)

                                     aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCtttt                                                                                                    >  1:2321221/1‑140 (MQ=255)

                                     aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCtttt                                                                                                    >  1:3258691/1‑140 (MQ=255)

                                     aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCttt                                                                                                     <  2:2009101/139‑1 (MQ=255)

                                        cGATGCGGTTTTGGTTGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg                                                                                                 <  2:1444720/140‑1 (MQ=255)

                                        cGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg                                                                                                 <  2:595204/140‑1 (MQ=255)

                                        cGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg                                                                                                 <  1:1711174/140‑1 (MQ=255)

                                          aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt                                                                                               <  1:665325/140‑1 (MQ=255)

                                            gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                                                             >  2:2140774/1‑140 (MQ=255)

                                            gCGGTTTTGGTCGTTGATTTGGCGGTGGATTTACGTGTAATACTGCTACTTTTCGCCTCACCCAGTTTTAGTTTCTTACCAACTTCAATGGTGTAAGGGGG‑GGGGAAGTGCGGTTTAGTCGCGCcttttcttttgggttg                                                                                              >  2:196213/1‑134 (MQ=255)

                                            gCGGTTTTGGTCGTTGATTTGGAGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg                                                                                             >  2:2578547/1‑140 (MQ=255)

                                                  ttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCg                                                                                       <  1:116834/140‑1 (MQ=255)

                                                    ggTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtg                                                                                     <  1:3042071/140‑1 (MQ=255)

                                                         ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                                                                <  1:365508/140‑1 (MQ=255)

                                                         ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                                                                <  2:3384489/140‑1 (MQ=255)

                                                         ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                                                                <  2:3345461/140‑1 (MQ=255)

                                                         ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg                                                                                <  2:3146919/140‑1 (MQ=255)

                                                          tGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcgc                                                                               >  2:1234704/1‑140 (MQ=255)

                                                          tGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcgc                                                                               >  1:185435/1‑140 (MQ=255)

                                                           gATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg                                                                              <  1:994002/140‑1 (MQ=255)

                                                             tttGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaa                                                                            <  2:672244/140‑1 (MQ=255)

                                                              ttGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa                                                                           <  2:1190811/140‑1 (MQ=255)

                                                               tGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAAt                                                                          <  1:158571/140‑1 (MQ=255)

                                                                ggaggTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                                         <  1:2578547/137‑1 (MQ=255)

                                                                ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                                         <  2:2614534/140‑1 (MQ=255)

                                                                ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa                                                                         <  1:2696088/140‑1 (MQ=255)

                                                                     ttGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGata                                                                    <  2:2321221/140‑1 (MQ=255)

                                                                            aCGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa                                                                           >  2:2269871/1‑126 (MQ=255)

                                                                            aCGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTa                                                             >  2:792389/1‑140 (MQ=255)

                                                                               tgtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcc                                                          <  2:521752/140‑1 (MQ=255)

                                                                                  aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccccc                                                       >  2:1398974/1‑140 (MQ=255)

                                                                                       tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcc                                                          <  2:3089841/132‑1 (MQ=255)

                                                                                       tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGttt                                                  >  1:1480132/1‑140 (MQ=255)

                                                                                        gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                                 <  2:2524196/140‑1 (MQ=255)

                                                                                        gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                                 >  2:1204111/1‑140 (MQ=255)

                                                                                        gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                                 <  1:2833537/140‑1 (MQ=255)

                                                                                        gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                                 <  1:520323/140‑1 (MQ=255)

                                                                                        gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                                 >  1:223858/1‑140 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc                                                 >  1:736959/1‑140 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGTCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                >  1:1020662/1‑140 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                >  2:993557/1‑140 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                <  2:1123185/140‑1 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                <  1:1398974/140‑1 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                <  1:2140774/140‑1 (MQ=255)

                                                                                         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa                                                >  1:2867077/1‑140 (MQ=255)

                                                                                          tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                               <  1:3064965/140‑1 (MQ=255)

                                                                                               ttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGgtgt                                          >  1:1465220/1‑140 (MQ=255)

                                                                                                 cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCGCGgt                                            >  1:1802786/1‑136 (MQ=255)

                                                                                                 cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCGCGgt                                            <  2:1802786/136‑1 (MQ=255)

                                                                                                  gCCCAACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaaa                                       <  2:41975/140‑1 (MQ=255)

                                                                                                    cccacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACg                                     <  2:575055/140‑1 (MQ=255)

                                                                                                      cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGa                                   <  1:2509397/140‑1 (MQ=255)

                                                                                                      cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGa                                   <  1:1468488/140‑1 (MQ=255)

                                                                                                            gTTTTAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGa                             <  1:1234704/140‑1 (MQ=255)

                                                                                                             ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGa                             <  1:63466/140‑1 (MQ=255)

                                                                                                             ttttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa                            <  1:2036560/140‑1 (MQ=255)

                                                                                                             ttttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa                            <  1:3352690/140‑1 (MQ=255)

                                                                                                               ttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                          <  2:2059655/140‑1 (MQ=255)

                                                                                                               ttAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                          <  1:52681/140‑1 (MQ=255)

                                                                                                               ttAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGGTGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                          <  1:3227331/140‑1 (MQ=255)

                                                                                                                tAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc                                 <  1:211213/132‑1 (MQ=255)

                                                                                                                tAGTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc                                 >  2:211213/1‑132 (MQ=255)

                                                                                                                  gTTTCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGt                       >  1:1939706/1‑140 (MQ=255)

                                                                                                                    ttCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc                     <  2:3056208/140‑1 (MQ=255)

                                                                                                                    ttCTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc                     <  2:223858/140‑1 (MQ=255)

                                                                                                                      cTGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCt                   >  2:487196/1‑140 (MQ=255)

                                                                                                                       tGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTg                  >  1:3309326/1‑140 (MQ=255)

                                                                                                                       tGACCAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTg                  >  1:2588128/1‑140 (MQ=255)

                                                                                                                       tGACCAACTTCAATGGTGTAA‑‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGt                 <  1:1199312/140‑1 (MQ=255)

                                                                                                                           cAACTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATc              <  1:1532360/140‑1 (MQ=255)

                                                                                                                             aCTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTa                >  1:580497/1‑136 (MQ=255)

                                                                                                                             aCTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCgg            <  2:1614250/140‑1 (MQ=255)

                                                                                                                             aCTTCAATGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCG‑AATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGga           >  1:2709075/1‑140 (MQ=255)

                                                                                                                                   aTGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCt                   >  2:3113867/1‑127 (MQ=255)

                                                                                                                                   aTGGTGTAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCt                   <  1:3113867/127‑1 (MQ=255)

                                                                                                                                       tgtAAG‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCt  >  1:1315103/1‑140 (MQ=255)

GCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑GGG‑GGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCT  >  NC_000913/2999534‑2999807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A