Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,200,184	A→G	V261A (GTC→GCC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,200,184	0	A	G	100.0%	165.1 / NA	48	V261A (GTC→GCC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/23); total (25/23)

GTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCC  >  NC_000913/3200055‑3200318

gtagagtagaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAAt                                                                                                                              >  2:750749/1‑140 (MQ=255)

     gtagaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTg                                                                                                                         >  2:378377/1‑140 (MQ=255)

       agaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGcc                                                                                                                       <  2:34953/140‑1 (MQ=255)

              cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGGTCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCt                                                                                                                >  2:721262/1‑140 (MQ=255)

              cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCt                                                                                                                >  1:1197583/1‑140 (MQ=255)

              cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCt                                                                                                                >  1:445416/1‑140 (MQ=255)

                tCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGt                                                                                                              >  2:971587/1‑140 (MQ=255)

                tCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGt                                                                                                              >  2:569215/1‑140 (MQ=255)

                            ccGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCa                                                                                                  <  2:1197583/140‑1 (MQ=255)

                               cAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCg                                                                                               >  2:1139579/1‑140 (MQ=255)

                               cAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCg                                                                                               <  1:569215/140‑1 (MQ=255)

                                  aaGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTg                                                                                            >  2:27774/1‑140 (MQ=255)

                                   aGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGc                                                                                           <  2:982013/140‑1 (MQ=255)

                                     cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcg                                                                                         >  2:880041/1‑140 (MQ=255)

                                     cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcg                                                                                         <  1:587041/140‑1 (MQ=255)

                                      aaTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgc                                                                                        >  1:1111226/1‑140 (MQ=255)

                                       aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                       >  2:119477/1‑140 (MQ=255)

                                       aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                       >  2:1177111/1‑140 (MQ=255)

                                       aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                       >  1:818642/1‑140 (MQ=255)

                                        tGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGt                                                                                      <  1:1139579/140‑1 (MQ=255)

                                           tCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTAcc                                                                                   <  1:971587/140‑1 (MQ=255)

                                           tCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTAcc                                                                                   <  1:392458/140‑1 (MQ=255)

                                             gCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                 <  2:445416/140‑1 (MQ=255)

                                             gCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                 <  2:860331/140‑1 (MQ=255)

                                               cTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCcaca                                                                               <  1:119477/140‑1 (MQ=255)

                                                tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAg                                                                              <  1:750749/140‑1 (MQ=255)

                                                tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAg                                                                              <  1:668953/140‑1 (MQ=255)

                                                  gCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGtt                                                                            <  2:1073055/140‑1 (MQ=255)

                                                     ttGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttctt                                                                         <  2:720806/140‑1 (MQ=255)

                                                              cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttcttc                                                                        >  2:6892/1‑132 (MQ=255)

                                                              cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttcttc                                                                        <  1:6892/132‑1 (MQ=255)

                                                                              gtgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGc                                                >  1:174075/1‑140 (MQ=255)

                                                                                  tCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCTACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGc                                            >  2:489451/1‑140 (MQ=255)

                                                                                  tCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGc                                            >  1:74515/1‑140 (MQ=255)

                                                                                       ttACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAg                                       >  1:677649/1‑140 (MQ=255)

                                                                                          cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                    <  1:721262/140‑1 (MQ=255)

                                                                                          cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                    <  1:126634/140‑1 (MQ=255)

                                                                                              gCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCt                                >  2:385171/1‑140 (MQ=255)

                                                                                                acaATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTc                              >  1:12092/1‑140 (MQ=255)

                                                                                                acaATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTc                              >  2:225772/1‑140 (MQ=255)

                                                                                                 caATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTcc                             <  2:1111226/140‑1 (MQ=255)

                                                                                                    tACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGc                          <  1:1177111/140‑1 (MQ=255)

                                                                                                       caccGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGcccc                       <  2:174075/140‑1 (MQ=255)

                                                                                                         ccGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCTCAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                     >  2:560678/1‑140 (MQ=255)

                                                                                                             aCAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTcc                 >  1:829166/1‑140 (MQ=255)

                                                                                                              cAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                >  2:285623/1‑140 (MQ=255)

                                                                                                                gCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCcaca              <  1:385171/140‑1 (MQ=255)

                                                                                                                          aTGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTg    <  2:12092/140‑1 (MQ=255)

                                                                                                                           tGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCT‑ACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGcc  >  1:155825/1‑140 (MQ=255)

GTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCC  >  NC_000913/3200055‑3200318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A