Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,015	T→C	intergenic (‑50/‑192)	ygiF ← / → ygiM	inorganic triphosphatase/putative signal transduction protein (SH3 domain)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,015	0	T	C	100.0%	191.3 / NA	55	intergenic (‑50/‑192)	ygiF/ygiM	inorganic triphosphatase/putative signal transduction protein (SH3 domain)
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/25); total (30/25)

CTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTG  >  NC_000913/3200879‑3201147

cTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAAt                                                                                                                                   >  2:65996/1‑140 (MQ=255)

cTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAAt                                                                                                                                   >  1:636520/1‑140 (MQ=255)

  cgccgcCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGc                                                                                                                                 >  1:418281/1‑140 (MQ=255)

   gccgccCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCg                                                                                                                                <  2:420867/140‑1 (MQ=255)

    ccgccCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCAATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGa                                                                                                                               <  2:599476/140‑1 (MQ=255)

        ccAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCtt                                                                                                                           >  1:23217/1‑140 (MQ=255)

           gCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCttttt                                                                                                                        <  1:410896/140‑1 (MQ=255)

             gTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTg                                                                                                                      <  1:312760/140‑1 (MQ=255)

                ttGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCa                                                                                                                   <  2:206685/140‑1 (MQ=255)

                  gagaTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGt                                                                                                                 <  2:503638/140‑1 (MQ=255)

                    gaTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAg                                                                                                               >  1:343218/1‑140 (MQ=255)

                     aTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGACCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGa                                                                                                              >  1:676672/1‑140 (MQ=255)

                      tGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCa                                                                                                                   <  2:437510/134‑1 (MQ=255)

                      tGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCa                                                                                                                   >  1:437510/1‑134 (MQ=255)

                        gTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAg                                                                                                           >  2:698654/1‑140 (MQ=255)

                          cACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTa                                                                                                         >  1:245582/1‑140 (MQ=255)

                                 cGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTgcg                                                                                                  <  1:698654/140‑1 (MQ=255)

                                   ccTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGcc                                                                                                >  1:832007/1‑140 (MQ=255)

                                   ccTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGcc                                                                                                >  2:774166/1‑140 (MQ=255)

                                               tgtgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGc                                                                                    <  2:245582/140‑1 (MQ=255)

                                                 tgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCGCATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCaa                                                                                  <  2:676672/140‑1 (MQ=255)

                                                 tgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCaa                                                                                  <  1:774166/140‑1 (MQ=255)

                                                 tgACTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCaa                                                                                  <  2:397183/140‑1 (MQ=255)

                                                           tAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAAttt                                                                        >  1:705026/1‑140 (MQ=255)

                                                           tAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAAttt                                                                        >  1:41859/1‑140 (MQ=255)

                                                               cTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACg                                                                    <  2:705026/140‑1 (MQ=255)

                                                                      tCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTaa                                                             <  2:832007/140‑1 (MQ=255)

                                                                                 aGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGc                                                  >  1:449095/1‑140 (MQ=255)

                                                                                      tGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTgg                                             >  2:729480/1‑140 (MQ=255)

                                                                                        gCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCa                                           <  1:729480/140‑1 (MQ=255)

                                                                                           tttACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGa                                        >  1:665746/1‑140 (MQ=255)

                                                                                             tACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGAcc                                      >  2:570699/1‑140 (MQ=255)

                                                                                               cTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTg                                    <  2:343218/140‑1 (MQ=255)

                                                                                                  aTCGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGaca                                 >  2:235746/1‑140 (MQ=255)

                                                                                                  aTCGGTTATGTCACATAAATGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGAATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGAGAGTAAAAGTTTGAAGCAGTGGCAAGACCTGaca                                 >  2:121783/1‑140 (MQ=255)

                                                                                                    cGGTTATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGAcaca                               <  2:295559/140‑1 (MQ=255)

                                                                                                       ttATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACagag                            >  1:79999/1‑140 (MQ=255)

                                                                                                       ttATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACagag                            <  2:733562/140‑1 (MQ=255)

                                                                                                       ttATGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACagag                            >  1:293054/1‑140 (MQ=255)

                                                                                                         aTGTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGa                          >  2:7193/1‑140 (MQ=255)

                                                                                                           gTCACATAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGAtt                        <  2:418281/140‑1 (MQ=255)

                                                                                                               caTAAGTGACGATGAGCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTGAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa                    <  1:340715/140‑1 (MQ=255)

                                                                                                               caTAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa                    <  1:157863/140‑1 (MQ=255)

                                                                                                                aTAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGAt                   >  2:296378/1‑140 (MQ=255)

                                                                                                                 tAAGTGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGAtt                  >  2:512449/1‑140 (MQ=255)

                                                                                                                     tGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg              >  1:613500/1‑140 (MQ=255)

                                                                                                                     tGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg              >  1:749898/1‑140 (MQ=255)

                                                                                                                     tGACGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg              >  1:797401/1‑140 (MQ=255)

                                                                                                                       aCGATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTc            >  1:396822/1‑140 (MQ=255)

                                                                                                                         gATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGa          <  2:636520/140‑1 (MQ=255)

                                                                                                                         gATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGa          <  2:449095/140‑1 (MQ=255)

                                                                                                                         gATGAACGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGa          >  2:756553/1‑140 (MQ=255)

                                                                                                                             aaCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGc      >  2:626830/1‑140 (MQ=255)

                                                                                                                              aCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCt     <  2:665746/140‑1 (MQ=255)

                                                                                                                              aCGGCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCt     >  1:471621/1‑140 (MQ=255)

                                                                                                                                 gCGAACTCAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTg  <  1:7193/140‑1 (MQ=255)

CTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTG  >  NC_000913/3200879‑3201147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A