Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,717,500	C→T	G170S (GGC→AGC)	pdxH ←	pyridoxal 5‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,717,500	0	C	T	98.5%	229.7 / ‑5.9	66	G170S (GGC→AGC)	pdxH	pyridoxal 5‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (31/34); minor base A (1/0); total (32/34)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCACGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTTCATCACTTC  >  NC_000913/1717373‑1717635

ttCCACGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACtt                                                                                                                             >  2:587055/1‑140 (MQ=255)

ttCCACGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACtt                                                                                                                             <  1:274369/140‑1 (MQ=255)

 tCCACGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTc                                                                                                                            >  1:1083251/1‑140 (MQ=255)

  ccACGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCt                                                                                                                           >  1:354899/1‑140 (MQ=255)

    aCGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGc                                                                                                                         >  1:388536/1‑140 (MQ=255)

     cGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCt                                                                                                                        >  1:1180664/1‑140 (MQ=255)

      gCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTAGCTCTGTTGAAACTTCTGCtt                                                                                                                       <  2:891788/140‑1 (MQ=255)

       catcatTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTc                                                                                                                      <  1:587055/140‑1 (MQ=255)

       catcatTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTc                                                                                                                      <  1:174642/140‑1 (MQ=255)

        atcatTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCGCTTCGCTCTGTTGAAACTTCTGCTTCa                                                                                                                     >  2:470711/1‑140 (MQ=255)

                  cGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTc                                                                                                                 >  2:532284/1‑134 (MQ=255)

                  cGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTc                                                                                                                 <  1:532284/134‑1 (MQ=255)

                  cGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGaa                                                                                                           >  2:822189/1‑140 (MQ=255)

                    cTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAAtt                                                                                                         <  2:354899/140‑1 (MQ=255)

                      ggTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTa                                                                                                       <  2:1083251/140‑1 (MQ=255)

                      ggTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTa                                                                                                       <  1:1224475/140‑1 (MQ=255)

                       gTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAATTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCACTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTAc                                                                                                      >  2:865920/1‑140 (MQ=255)

                       gTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTAc                                                                                                      >  1:387035/1‑140 (MQ=255)

                       gTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTAc                                                                                                      >  2:1176472/1‑140 (MQ=255)

                        tACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACt                                                                                                     <  2:151783/140‑1 (MQ=255)

                                gCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGa                                                                                             <  2:911104/140‑1 (MQ=255)

                                 cGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTa                                                                                                       <  2:62646/129‑1 (MQ=255)

                                 cGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTa                                                                                                       >  1:62646/1‑129 (MQ=255)

                                 cGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGAt                                                                                            >  1:457941/1‑140 (MQ=255)

                                 cGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGAt                                                                                            >  1:455284/1‑140 (MQ=255)

                                 cGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGAt                                                                                            >  2:100894/1‑140 (MQ=255)

                                  ggTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATa                                                                                           >  2:488531/1‑140 (MQ=255)

                                  ggTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATa                                                                                           >  2:88070/1‑140 (MQ=255)

                                     cATGCAGGCGATGCTCACCCCCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACcg                                                                                        <  2:1257264/140‑1 (MQ=255)

                                         cAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCggg                                                                                    <  1:1176472/140‑1 (MQ=255)

                                          aGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGc                                                                                   <  1:822189/140‑1 (MQ=255)

                                              gATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGaa                                                                               >  2:1262391/1‑140 (MQ=255)

                                               aTGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGaaa                                                                              <  2:388536/140‑1 (MQ=255)

                                                    caccacCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCga                                                                         <  1:88070/140‑1 (MQ=255)

                                                          ccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCgactggac                                                                   >  1:899315/1‑140 (MQ=255)

                                                          ccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCgactggac                                                                   <  1:865920/140‑1 (MQ=255)

                                                          ccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCgactggac                                                                   >  2:837502/1‑140 (MQ=255)

                                                          ccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCgactggac                                                                   <  1:558223/140‑1 (MQ=255)

                                                                  aaCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGa                                                           <  2:899315/140‑1 (MQ=255)

                                                                   aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCgactggactg                                                                 <  1:799053/133‑1 (MQ=255)

                                                                   aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCgactggactg                                                                 >  2:799053/1‑133 (MQ=255)

                                                                       aaTCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCGCTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAAccc                                                      <  1:470711/140‑1 (MQ=255)

                                                                       aaTCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAAccc                                                      <  1:584170/140‑1 (MQ=255)

                                                                                 aGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGc                                                  <  2:923266/134‑1 (MQ=255)

                                                                                 aGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGc                                                  >  1:923266/1‑134 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCTAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCa                                                 >  1:1100435/1‑129 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAAccc                                                      <  2:410802/124‑1 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAAccc                                                      >  1:410802/1‑124 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCa                                                 <  2:1191811/129‑1 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCa                                                 <  2:1100435/129‑1 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCa                                                 >  1:1191811/1‑129 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCt                                      >  1:1109927/1‑140 (MQ=255)

                                                                                       aCGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCt                                      >  1:288493/1‑140 (MQ=255)

                                                                                                   ccccAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACgcgggcg                          >  2:37994/1‑140 (MQ=255)

                                                                                                   ccccAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACgcgggcg                          >  1:345487/1‑140 (MQ=255)

                                                                                                     ccAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGggcggc                        <  1:37994/140‑1 (MQ=255)

                                                                                                      cAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCt                       <  1:488531/140‑1 (MQ=255)

                                                                                                      cAAAAGCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCt                       <  1:100894/140‑1 (MQ=255)

                                                                                                           gCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGaa                  <  1:1262391/140‑1 (MQ=255)

                                                                                                           gCTCGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGaa                  <  2:90737/140‑1 (MQ=255)

                                                                                                              cGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGaaaa                >  1:357575/1‑139 (MQ=255)

                                                                                                              cGGCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGaaaa                <  2:357575/139‑1 (MQ=255)

                                                                                                               ggCAATGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAata              >  1:979271/1‑140 (MQ=255)

                                                                                                                   aTGGCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTtc          <  2:979271/140‑1 (MQ=255)

                                                                                                                     ggCACTTCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTtcat        <  2:387035/140‑1 (MQ=255)

                                                                                                                           tCGCTCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTTCATCACTTc  >  2:1061307/1‑140 (MQ=255)

TTCCACGCATCATTTTCACGCTGGTACAAAAAGCGGTCATGCAGGCGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTTCATCACTTC  >  NC_000913/1717373‑1717635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A